Results 31 to 40 of about 153,070 (262)

Hypoxia-induced mitochondrial stress granules

open access: yesCell Death and Disease, 2023
Perturbations of mitochondrial proteostasis have been associated with aging, neurodegenerative diseases, and recently with hypoxic injury. While examining hypoxia-induced mitochondrial protein aggregation in C.
Chun-Ling Sun   +2 more
doaj   +1 more source

Highly Sensitive Detection of DNA Fragments by an Inexpensive and Simple Method Fabricated In-House Blue LED Transilluminator

open access: yesGenetics & Applications, 2023
Agarose gel electrophoresis is a routinely employed experimental technique for visualizing nucleic acids, typically combined with ethidium bromide or some other molecular dye staining and a UV transilluminator.
Ryosuke Ishii
doaj   +1 more source

Efflux of ethidium bromide.

open access: yes, 2019
Bacteria were treated with ethidium bromide and CCCP for 60 min and then re-energized with glucose. Data presented is the time taken for the fluorescence to decrease by 25% +/- SE. (Data for 10% and 50% drop can be seen in S1 Table). A.
Matthew Davies (247938)   +11 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Glucose Deprivation‐Driven Concurrent Induction of Apoptosis/Disulfidptosis/Ferroptosis by Tumor Targeting Nanoassemblies in Triple‐Negative Breast Cancer Therapy

open access: yesAdvanced Functional Materials, EarlyView.
Innovative hybrid nanoassemblies installed with ferrocene, D‐α‐tocopherol succinate, and phloretin exploit the metabolic vulnerability of triple‐negative breast cancer under glucose starvation. By blocking glucose uptake, this single nanoplatform simultaneously orchestrates apoptosis, disulfidptosis, and ferroptosis to achieve synergistic and ...
Song Yi Lee   +10 more
wiley   +1 more source

Thermally Modulated Metasurface Sensor for Dynamic and Time‐Resolved Isolation of Extracellular Vesicles

open access: yesAdvanced Materials, EarlyView.
A thermally adaptive plasmonic metasurface, functionalized with poly(N‐isopropylacrylamide) (PNIPAM) and anti‐CD63 antibodies, enables precise, on‐demand capture and release of extracellular vesicles (EVs). Delivering high recovery, improved purity, and intact vesicle structure, the platform offers a versatile, label‐free solution for real‐time EV ...
Beyza Nur Kucuk   +7 more
wiley   +1 more source

Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy

open access: yesAnnals of Clinical Microbiology and Antimicrobials, 2005
Changes in morphology and growth curve of Candida albicans in response to treatment by Cisplatin has been studied using fluorescence staining with ethidium bromide.
Ganesan Natarajan   +2 more
doaj   +1 more source

Ethidium bromide exclusion assay.

open access: yes, 2013
Release of ethidium bromide (EB) from DNA–EB complexes upon addition of each cationic cholesterol gemini lipid suspension at different lipid/DNA charge ratios.
Paturu Kondaiah (73230)   +3 more
core   +1 more source

Additive‐Manufactured, Multifunctional Bioreactor Technology for Dynamic Culture of 3D Bioprinted Tissue Models

open access: yesAdvanced Materials Technologies, EarlyView.
We introduce an additively manufactured bioreactor with a perfusion flow system and integrated temperature and pH sensors for skeletal muscle tissue biofabrication. The bioreactor's performance was evaluated by assessing the viability, spreading of the myoblast cells in a printed scaffold, and contraction of the isolated murine musculi interossei ...
Lys Sprenger   +9 more
wiley   +1 more source

Digested bands detected with non-denaturing polyacrylamide gels stained with either silver (a) or ethidium bromide (b) and agarose gels stained with ethidium bromide (c).

open access: yes, 2013
Putative mutations in the pools (1, 2, 3, 4) are identified by the presence of two bands (indicated by white arrows), with sizes adding up to the full length PCR product. (a). Non-denaturing polyacrylamide gel stained with silver; (b).
Andy Phillips (11704)   +7 more
core   +1 more source

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