Results 81 to 90 of about 153,070 (262)

Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

open access: yesBioTechniques, 2007
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However, it has been essentially forgotten in DNA quantification by spectrofluorimetry.
Veronica Bonasera   +2 more
doaj   +1 more source

Prevalence and Genomic Characterization of mcr‐Positive Enterobacteriaceae in Retail Meat in Thailand Following the Colistin Ban

open access: yesMicrobiology and Immunology, EarlyView.
ABSTRACT This study aimed to investigate the prevalence and characteristics of mcr‐positive Enterobacteriaceae in retail meat in Thailand following the national ban on prophylactic colistin use in food producing animals. A total of 152 meat samples (103 chicken and 49 pork) were collected from supermarkets and open markets between July and September ...
Ahmed Abououf   +7 more
wiley   +1 more source

A Novel Antioxidant Peptide Cath‐Np Derived from Nanorana parkeri Attenuates UVB‐Induced Skin Photodamage in Mice

open access: yesIntegrative Zoology, EarlyView.
Schematic representation of Cath‐Np as an antioxidant suppressing acute skin damage caused by ultraviolet B (UVB) radiation in mice. Cath‐Np inhibits the activation of p53 and mitogen‐activated protein kinase pathways while it activates the Nrf2 pathway by its strong antioxidant effects, and accordingly suppresses the expression of inflammatory factors
Hang Liao   +8 more
wiley   +1 more source

Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

open access: yesGenetics and Molecular Biology, 2009
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence
Cintya A. Christofoletti   +2 more
doaj  

Ameliorating the Effects of Trypanosoma Evansi Infection in Wistar Rats Using Selected Nutraceuticals, Ethidium Bromide and Ethidium Chloride

open access: yesUMYU Scientifica Journal
African Trypanosomiasis is a disease that affects humans and animals, caused by trypanosomes in sub-Saharan Africa, despite decades of vector control and treatments. The disease remains endemic and an impediment to socio-economic development.
Yahaya Shuaibu   +2 more
doaj   +1 more source

Quantification of DNA samples by Ethidium Bromide Spot Technique

open access: yes, 2018
Accurate and quick determination of DNA concentration is critical for the assembly of synthetic constructs, as well as a multitude of other experiments. We sought to optimize an under-utilized and inexpensive approach for determining DNA concentration: a
Antoine Cummins   +5 more
core   +1 more source

Conservation implications of low contemporary connectivity along the Mid‐Atlantic Ridge in hydrothermal vent gastropods

open access: yesConservation Biology, EarlyView.
Abstract Polymetallic sulfide deposits produced at hydrothermal vent fields are targets for mining exploitation along the Mid‐Atlantic Ridge, threatening the functioning and resilience of vent ecosystems that provide multiple ecosystem services. Knowledge about connectivity between vents will inform conservation practices.
E. Portanier   +10 more
wiley   +1 more source

Uroporphyria development in cultured chick embryo fibroblasts long-term treated with chloramphenicol and ethidium bromide

open access: yes, 1984
Long-term chloramphenicol- and ethidium bromide-treated chick embryo fibroblasts synthesize large amounts of porphyrins from exogenously added σ-aminolevulinic acid. The porphyrins consist mainly of uro- and heptacarboxyporphyrins and are retained within
de Muys, Jean-Marc   +3 more
core   +1 more source

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