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Destruction of Ethidium Bromide in Solution by Ozonolysis
Biological Chemistry Hoppe-Seyler, 1988Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic.
Petra Messner +3 more
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Inhibition of yeast sporulation by ethidium bromide
Molecular and General Genetics MGG, 1978Ethidium bromide blocks ascus formation in the yeast Saccharomyces cerevisiae. This may mean that the presence of the mitochondrial genome is required for sporulation in this organism.
Benjamin D. Hall, Michael C. Newlon
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Crystal structure of ethidium bromide
Journal of Crystal and Molecular Structure, 1971Crystals of ethidium bromide (2,7-diamino-9-phenyl-10-ethylphenanthridinium bromide) monohydrate are monoclinic,a = 9·577,b = 10·698,c = 20·242 A, β = 106·34 °,Z = 4, space groupP21/c. The structure was determined with CuKα. diffractometer data by direct methods, and was refined by full-matrix least-squares methods toR = 0·058 for 2151 observed ...
James Trotter +2 more
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Decontamination of Ethidium Bromide Spills
Applied Industrial Hygiene, 1989Abstract Samples of stainless steel, Formica, glass, and vinyl floor tile and the filters of transilluminators were contaminated with ethidium bromide and cleaned either by wiping once with a paper towel soaked in an aqueous solution of sodium nitrite and hypophosphorous acid followed by five wipes with wet towels or by wiping six times with only wet ...
Eric B. Sansone, George Lunn
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An evaluation of the ethidium bromide microcytotoxicity test
Journal of Immunological Methods, 1974Abstract An ethidium bromide fluorescence technique for the demonstration of lymphocytotoxic antibodies is described and evaluated. This fluorescence technique offers the advantages of speed, simplicity, high reproducibility and sensitivity.
C, Darke, E, Gay
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Staining of Nuclei in Fungi by Ethidium Bromide
Biotechnic & Histochemistry, 1991Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
U. S. Singh, J. Kumar
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Renaturation of DNA in the presence of ethidium bromide
Biopolymers, 1972AbstractThe rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75°C and for native DNA at 65°C in 0.4M Na+.
James R. Huttpm, James G. Wetmur
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Influence of ethidium bromide on respiration in Schizosaccharomyces pombe [PDF]
Molecular and General Genetics MGG, 1971 In Schizosaccharomyces pombe ethidium bromide inhibits respiratory competence in all treated cells. After inoculation in ethidium bromide free medium these cells show reversion to respiration competence.
M. Sebald, R. Schwab, F. Kaudewitz
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Yeast mutants resistant to ethidium bromide
Molecular and General Genetics MGG, 1973Yeast mutants resistant to ethidium bromide have been isolated among sensitive grande cells (ϱ+) for their ability to grow on glycerol in the presence of the dye. Mutant cells are also resistant to acriflavin and do not yield petites (ϱ-) when grown on galactose with the mutagen.
M Gouhier, Jean-Claude Mounolou
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Beiträge zur Pathologie, 1976
Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity
Roger Bassleer +2 more
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Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity
Roger Bassleer +2 more
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