Results 61 to 70 of about 239,329 (263)

Lean ZSF1 rats in basic research on heart failure with preserved ejection fraction

ESC Heart Failure, Volume 12, Issue 2, Page 1474-1478, April 2025.
Abstract Aims ZSF1 obese rats harbouring two mutant leptin receptor alleles (Leprcp and Leprfa) develop metabolic syndrome and heart failure with preserved ejection fraction (HFpEF), making them a widely used animal model in cardiometabolic research.
Petra Büttner, Antje Augstein, Mahmoud Abdellatif, André Lourenço, Adelino Leite‐Moreira, Inês Falcão‐Pires, Sarah Werner, Holger Thiele, Simon Sedej, Antje Schauer, Volker Adams   +10 more
wiley   +1 more source

DNA extraction from formalin-fixed tissue: new light from the deep sea

Scientia Marina, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds, Sven Thatje   +5 more
doaj   +1 more source

Single Cell Analysis of Human RAD18-Dependent DNA Post-Replication Repair by Alkaline Bromodeoxyuridine Comet Assay [PDF]

, 2013
Damage to DNA can block replication progression resulting in gaps in the newly synthesized DNA. Cells utilize a number of post-replication repair (PRR) mechanisms such as the RAD18 controlled translesion synthesis or template switching to overcome the ...
Downes, C. Stephen, Gali, Himabindu, Haracska, Lajos, Mórocz, Mónika, Raskó, István   +4 more
core   +2 more sources

Long‐lasting remodeling of astrocytes in an Scna1+/− mouse model of Dravet syndrome

Epilepsia, EarlyView.
Abstract Objective Dravet syndrome (DS) is a prototypical developmental and epileptic encephalopathy caused by mutations in the SCN1A gene, leading to loss of function of the voltage‐gated sodium channel Naᵥ1.1. The latter causes early onset drug‐resistant seizures and enduring cognitive and behavioral deficits.
Athénaïs Genin, Alicia Janvier, Tristan Moujellil‐Legagneur, Marine Blaquière, Alexis Chaussy, Romane Privé, Fabrice Duprat, Massimo Mantegazza, Etienne Audinat, Nicola Marchi, Noémie Cresto   +10 more
wiley   +1 more source

Health Biomarkers of Pesticide Exposure in Rural Populations of Santa Fe, Argentina: Oxidative Stress and Genotoxicity

Journal of Applied Toxicology, EarlyView.
ABSTRACT The present study aimed to quantify pesticide residues in environmental matrices and plasma and to evaluate their potential effects on oxidative stress and DNA damage in rural populations of Santa Fe province. Soil and rainwater samples were collected, and biological samples and semistructured surveys were obtained from volunteers in three ...
María Priscila Dechiara, Carlina Leila Colussi, Lucía Magdalena Odetti, Damián Marino, María Fernanda Simoniello   +4 more
wiley   +1 more source

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Antibiotics, 2020
Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating ...
Ray S. Almeida, Priscilla R. Freitas, Ana Carolina J. Araújo, Irwin R. Alencar Menezes, Eduardo L. Santos, Saulo R. Tintino, Talysson F. Moura, Jaime R. Filho, Vitória A. Ferreira, Ana Cristina A. Silva, Luiz E. Silva, Wanderlei do Amaral, Cícero Deschamps, Marcello Iriti, Henrique D. Melo Coutinho   +14 more
doaj   +1 more source

Evaluation of DNA/Chromosome Integrity and Cell Death in Human Metabolically Noncompetent and Competent Cells Exposed to N′‐(3,5‐Difluorobenzylidene)Pyridine‐4‐Carbohydrazide

Journal of Applied Toxicology, EarlyView.
ABSTRACT The N‐acylhydrazone scaffold is recognized as a privileged structure for the design of bioactive substances with increasing applications in medicinal chemistry research. Ensuring the safety of newly developed molecules is a critical step for both human health and environmental protection. Accordingly, this study aimed to evaluate the cytotoxic
Larissa Ribeiro Canuto Santos, Jéssica Natalia Barbosa de Almeida, Camila Cavalleiro Frias, Wanda Pereira Almeida, Edson Luis Maistro   +4 more
wiley   +1 more source

Investigating the Removal Ethidium Bromide from Aqueous Solutions using Activated Persulfate in Electrochemical Process

پژوهان, 2018
Background & Objectives: Ethidium bromide is known as a Mutagenic substance that due to some of its characteristics including accumulative property in organism bodies, can cause major environmental problems.
Mohammad Reza Samarghandi, Yousef Poureshgh, Mohammad Vanaei Tabar, Zahra Rahmani, Sattar Ahmadi   +4 more
doaj  

Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

Bangladesh Journal of Pharmacology, 2019
An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively ...
Malarvizhi Deivasigamani, Ajay Kasivishwanathan Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan   +4 more
doaj   +1 more source

Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyes [PDF]

, 1982
Subnanosecond time‐resolved fluorescence depolarization has been used to monitor the reorientation of ethidium bromide intercalated in native DNA, synthetic polynucleotide complexes, and in supercoiled plasmid DNA.
Millar, D. P., Robbins, R. J., Zewail, A. H.   +2 more
core