Results 11 to 20 of about 27,732 (116)
Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose. [PDF]
PLoS ONE, 2014 We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts.
Hirokazu Takahashi +10 more
doaj +1 more source
BMC Genomics, 2021 Background Trichophyton mentagrophyte (TM), a zoonotic pathogen, has been endangering public health due to emerging drug resistance. Although increased attention is paid to this issue, there is very limited research available on drug resistance in TM. In
Chenwen Xiao +11 more
doaj +1 more source
THE IMPACT OF THE GENETIC FACTOR IN THE REALIZATION OF THE ARTERIAL HYPERTENSION AND METABOLIC DISORDERS AT CHILDREN WITH OVERWEIGHT OR OBESITY [PDF]
Romanian Journal of Pediatrics, 2014 Essential arterial hypertension is a multifactor pathology and in its realization genetic and environmental factors play a very important role. It is estimated that 30-60% of the variation of the arterial hypertension among people is due to genetic ...
Svetlana Cojocari +3 more
doaj +1 more source
An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
BioTechniques, 2009 A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study.
Marita C. Barth +2 more
doaj +1 more source
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1998 Liposomes can be used for the intracellular delivery of drugs into macrophages. Previously, we developed a liposome-mediated macrophage 'suicide' technique based on the intraphagocytic accumulation of the liposomally delivered bisphosphonate clodronate. Later we found that the diamidine propamidine is even more effective in this approach.
Bakker, Jeroen +2 more
openaire +2 more sources
Bangladesh Journal of Pharmacology, 2019 An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively ...
Malarvizhi Deivasigamani +4 more
doaj +1 more source
Simple and efficient way to detect small polymorphic bands in plants
Genomics Data, 2015 There are many ways to detect polymorphism. In this study we use the microsatellite markers to detect the polymorphism for the salt tolerance. This method has been successfully conducted in Oryza sativa and Brassica juncea.
Manu Kumar +3 more
doaj +1 more source
A multidrug ABC transporter with a taste for salt.
PLoS ONE, 2009 BackgroundLmrA is a multidrug ATP-binding cassette (ABC) transporter from Lactococcus lactis with no known physiological substrate, which can transport a wide range of chemotherapeutic agents and toxins from the cell. The protein can functionally replace
Saroj Velamakanni +14 more
doaj +1 more source
Advanced Science, EarlyView.ABSTRACT Vascularization remains a major obstacle in tissue engineering. Here, we introduce a bioprinting strategy to generate centimeter‐scale, self‐organizing “mother vessel” constructs from iPSC‐derived hiMPCs. By optimizing bioink composition, printing was accomplished in a single‐step approach. Within one week, hiMPCs differentiated into both CD31+
Leyla E. Dogan +5 more
wiley +1 more source
Advanced Science, EarlyView.Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak +6 more
wiley +1 more source