Results 191 to 200 of about 25,451,695 (279)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Safety evaluation of the food enzyme pectinesterase from the non-genetically modified <i>Aspergillus luchuensis</i> strain AP2 903-6. [PDF]

EFSA J
EFSA Panel on Food Enzymes (FEZ), Zorn H, Barat Baviera JM, Bolognesi C, Catania F, Gadermaier G, Greiner R, Mayo B, Mortensen A, Roos YH, Solano MLM, Van Loveren H, Vernis L, Andreoli C, Terron A, Yurkov A, Aguilera J, Andryszkiewicz M, Cavanna D, Criado A, Liu Y.   +20 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Safety of cannabidiol isolate from <i>Cannabis sativa</i> L. as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Bohn T, Cámara M, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Cubadda F, McArdle HJ, Trezza V, Albert O, Beneventi E, Laganaro M, Nuin Garciarena I, Rossi A, Berrío Cruz S, Seminati F, Magani M, Hirsch-Ernst KI.   +26 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Scientific opinion on the modification of the terms of authorisation of the preparations of <i>Lactiplantibacillus plantarum</i>CNCM I-3235, <i>Pediococcus acidilactici</i>CNCM I-3237, <i>Pediococcus pentosaceus</i>NCIMB 12455, <i>Acidipropionibacterium acidipropionici</i>CNCM I-4661 and <i>Lentilactobacillus hilgardii</i>CNCM I-4785 as feed additives for all animal species (Danstar Ferment AG). [PDF]

EFSA J
EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Villa RE, Azimonti G, Bonos E, Christensen H, Durjava M, Dusemund B, Gehring R, Glandorf B, Kouba M, López-Alonso M, Marcon F, Nebbia C, Pechová A, Prieto-Maradona M, Theodoridou K, Anguita M, Brozzi R, Galobart J, Sandalli S.   +19 more
europepmc   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

DNA-binding activity of PIF7 links phytochrome B signaling to plant responses to vegetation proximity. [PDF]

Plant Physiol
Diaz-Lopez M, Pastor-Andreu P, Qin W, Sanchez-Garcia A, Burbano-Erazo E, Valverde L, Paulišić S, Rodriguez-Concepcion M, Moreno-Romero J, Martinez-Garcia JF.   +9 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Rapid and Highly Efficient CRISPR-Cas9 RNP Genome Editing in Primary ILC2s. [PDF]

Eur J Immunol
Candelotti AM, Garzillo G, Bartolini S, Di Censo C, Peruzzi G, Stabile H, Fionda C, Sciumè G, Pietropaolo G.   +8 more
europepmc   +1 more source