Results 161 to 170 of about 1,401,784 (268)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Barriers to integrating evidence-based practices into intrapartum care during vaginal births: a descriptive qualitative study in Sri Lanka. [PDF]

BMJ Open
Weerasingha TK, Ratnayake C, Rathnayake A, Tennakoon SUB.   +3 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Expansion of Personas to Guide Professional Development in Implementing Evidence-Based Practices in Diverse Institutions. [PDF]

CBE Life Sci Educ
Dunbar-Wallis AK, Zagallo P, Meeder A, Schwartz Z, Groenevelt E, Bilinski T, Jaeger D, Corwin L.   +7 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

How do health professionals face barriers? A quantitative approach to the adoption of evidence-based practices in mental health care. [PDF]

Glob Ment Health (Camb)
Félix-Romero V, Rosas-Peña M, Tzek-Salazar DP, Martínez-Martínez KI, Morales-Chainé S.   +4 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Evidence-Based Serious Games in Pediatric Care

Children may find hospitalization to be a stressful experience. Children who are hospitalized show high levels of anxiety symptoms. Being away from home and entering a hospital environment causes anxiety and stress for both the child and the child's ...
Sert, H.P.
core  

Implementing Evidence-Based Practices in Hospitals: A Narrative Review of Implementer Roles and Project Attributes. [PDF]

Nurs Res Pract
Arsenault Knudsen ÉN, Krainak K, Ruona M.   +2 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source