Results 101 to 110 of about 1,214,634 (346)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Phenomenology of retained refractoriness: On semi-memristive discrete media

, 2011
We study two-dimensional cellular automata, each cell takes three states: resting, excited and refractory. A resting cell excites if number of excited neighbours lies in a certain interval (excitation interval).
Adamatzky A., Adamatzky A., Adamatzky A., Adamatzky A., ANDREW ADAMATZKY, LEON O. CHUA, Yang J. J.   +6 more
core   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Ultrafast Two‐Color X‐Ray Emission Spectroscopy Reveals Excited State Landscape in a Base Metal Dyad

Advanced Science
Effective photoinduced charge transfer makes molecular bimetallic assemblies attractive for applications as active light‐induced proton reduction systems. Developing competitive base metal dyads is mandatory for a more sustainable future.
Michal Nowakowski, Marina Huber‐Gedert, Hossam Elgabarty, Aleksandr Kalinko, Jacek Kubicki, Ahmet Kertmen, Natalia Lindner, Dmitry Khakhulin, Frederico A. Lima, Tae‐Kyu Choi, Mykola Biednov, Lennart Schmitz, Natalia Piergies, Peter Zalden, Katharina Kubicek, Angel Rodriguez‐Fernandez, Mohammad Alaraby Salem, Sophie E. Canton, Christian Bressler, Thomas D. Kühne, Wojciech Gawelda, Matthias Bauer   +21 more
doaj   +1 more source

Engineering bright J-aggregates through manipulation of electron acceptor for in vivo NIR-II fluorescence imaging

Journal of Innovative Optical Health Sciences
Fluorophores emitting in the second near-infrared window (NIR-II, 900–1700[Formula: see text]nm) allow for high-resolution deep-tissue bioimaging owing to minimal tissue scattering.
Yonghui Pan, Xianwei Weng, Mingxuan Jia, Xiaofei Miao, Hui Zhao, Jie Zhang, Wenbo Hu, Quli Fan   +7 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Tuning the Inter-Chromophore Electronic Coupling in Perylene Diimide Dimers with Rigid Covalent Linkers

Molecules
The organic multi-chromophore system has been increasingly attractive due to the potential optoelectronic applications. The inter-chromophore electronic coupling (EC), i.e., JCoul and JCT, plays a critical role in determining the relaxation path of the ...
Guo Yu, Yixuan Gao, Yonghang Li, Yiran Tian, Xiaoyu Zhang, Yandong Han, Jinsheng Song, Wensheng Yang, Xiaonan Ma   +8 more
doaj   +1 more source

Design and Analysis of Dual-Rotor Modular-Stator Hybrid-Excited Axial-Flux Permanent Magnet Vernier Machine

, 2022
Lun Jia, Mingyao Lin, Keman Lin, Le Wei, Anchen Yang   +4 more
openalex   +2 more sources

A new approach for Delta form factors

, 2010
We discuss a new approach to reducing excited state contributions from two- and three-point correlation functions in lattice simulations. For the purposes of this talk, we focus on the Delta(1232) resonance and discuss how this new method reduces excited
Aubin, C., Orginos, K.
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source