The Sm complex is required for the processing of non-coding RNAs by the exosome. [PDF]
A key question in the field of RNA regulation is how some exosome substrates, such as spliceosomal snRNAs and telomerase RNA, evade degradation and are processed into stable, functional RNA molecules.
Sarah Coy+3 more
doaj +5 more sources
The Pyrococcus Exosome Complex [PDF]
The exosome is a conserved eukaryotic enzymatic complex that plays an essential role in many pathways of RNA processing and degradation. Here, we describe the structural characterization of the predicted archaeal exosome in solution using small angle x-ray scattering. The structure model calculated from the small angle x-ray scattering pattern provides
Celso Raul Romero Ramos+3 more
openalex +5 more sources
CRABP1-complexes in exosome secretion
Background Cellular retinoic acid binding protein 1 (CRABP1) mediates rapid, non-canonical activity of retinoic acid (RA) by forming signalosomes via protein-protein interactions.
Jennifer Nhieu+4 more
doaj +3 more sources
The Rrp4-exosome complex recruits and channels substrate RNA by a unique mechanism. [PDF]
Cvetkovic MA+4 more
europepmc +3 more sources
A complex prediction: three‐dimensional model of the yeast exosome [PDF]
We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the
Patrick Aloy+7 more
openalex +7 more sources
Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome [PDF]
In mammalian cells, spurious transcription results in a vast repertoire of unproductive non-coding RNAs, whose deleterious accumulation is prevented by rapid decay. The nuclear exosome targeting (NEXT) complex plays a central role in directing non-functional transcripts to exosome-mediated degradation, but the structural and molecular mechanisms remain
Gerlach, Piotr+7 more
openaire +2 more sources
Post-transcriptional control of cellular differentiation by the RNA exosome complex. [PDF]
Abstract Given the complexity of intracellular RNA ensembles and vast phenotypic remodeling intrinsic to cellular differentiation, it is instructive to consider the role of RNA regulatory machinery in controlling differentiation.
Fraga de Andrade I+2 more
europepmc +5 more sources
CryoEM structure of yeast cytoplasmic exosome complex. [PDF]
The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway.
Liu JJ+14 more
europepmc +5 more sources
The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex [PDF]
The Ski complex is a conserved multiprotein assembly required for the cytoplasmic functions of the exosome, including RNA turnover, surveillance, and interference. Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2 stoichiometry. The crystal structure of an S. cerevisiae 370 kDa core complex shows that Ski3 forms an array of 33 TPR motifs organized
Felix Halbach+3 more
openalex +3 more sources
Autoantibodies directed to novel components of the PM/Scl complex, the human exosome [PDF]
The autoantigenic polymyositis/scleroderma (PM/Scl) complex was recently shown to be the human homologue of the yeast exosome, which is an RNA-processing complex. Our aim was to assess whether, in addition to targeting the known autoantigens PM/Scl-100 and PM/Scl-75, autoantibodies also target recently identified components of the PM/Scl complex.
Rick Brouwer+10 more
openalex +6 more sources