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Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus
Biochimie, 2012The archaeal exosome is a protein complex involved in the degradation and the posttranscriptional tailing of RNA. The proteins Rrp41, Rrp42, Rrp4, Csl4 and DnaG are major subunits of the exosome in Sulfolobus solfataricus. In vitro, Rrp41 and Rrp42 form a catalytically active hexamer, to which an RNA-binding cap of Rrp4 and/or Csl4 is attached.
Verena Roppelt +4 more
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Comparison of the yeast and human nuclear exosome complexes
Biochemical Society Transactions, 2012Most RNAs in eukaryotic cells are produced as precursors that undergo processing at the 3′ and/or 5′ end to generate the mature transcript. In addition, many transcripts are degraded not only as part of normal recycling, but also when recognized as aberrant by the RNA surveillance machinery.
Claudia Schneider +2 more
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In Vitro Characterization of the Prokaryotic Counterparts of the Exosome Complex
2019The same basic set of enzymatic activities exhibited by the eukaryotic RNA exosome are also found in prokaryotes. Bacteria have two predominant and distinct 3'→5' exoribonuclease activities: one is characterized by processive hydrolysis, derived from RNase II and RNase R, and the other by processive phosphorolysis, derived from PNPase.
Rute G. Matos +2 more
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Exosome complex and pervasive transcription in eukaryotic genomes
Current Opinion in Cell Biology, 2009Exosome complex is widely conserved, functionally versatile, and essential constituent of the machinery regulating gene expression in the nucleus as well as in the cytoplasm. While the most fundamental enzymatic property of exosome is ribonucleolytic activity, its in vivo functions are varied, highly specific, and tightly regulated, and include RNA ...
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Proteomic Analysis of Yeast Mutant RNA Exosome Complexes
Journal of Proteome Research, 2013The yeast exosome is a conserved multiprotein complex essential for RNA processing and degradation. The complex is formed by a nine-subunit core that associates with two hydrolytic 3'-5' exoribonucleases. Although catalytically inert, the assembly of this nine-subunit core seems to be essential for the exosome activity, as mutations in regions that do ...
Rogério F. Lourenço +2 more
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Chapter 21 Transcriptome Targets of the Exosome Complex in Plants
2008The exosome complex is endowed with the capabilities to conduct 3'-end RNA processing, 3'-end degradation, and surveillance of various RNA substrates. Although the exosome is present in both eukaryotes and archaea, where it plays a central role in cellular RNA metabolism, a substantial degree of variability exists in its modus operandi in the different
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RNA degradation paths in a 12-subunit nuclear exosome complex
Nature, 2015The eukaryotic exosome is a conserved RNA-degrading complex that functions in RNA surveillance, turnover and processing. How the same machinery can either completely degrade or precisely trim RNA substrates has long remained unexplained. Here we report the crystal structures of a yeast nuclear exosome containing the 9-subunit core, the 3'-5' RNases ...
Benjamin Schuch +5 more
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NMR studies of the archaeal exosome complex
2016This study is about structural studies of the exosome from Sulfolobus solfataricus. It includes dynamics analysis (by using Nuclear Magnetic Resonance) of the complex in absence and in presence of RNA. Additional biochemical experiments (degradation assays, fluorescence anisotropy) allowed further description of the interaction exosome-RNA.
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The Role of Aptamer Loaded Exosome Complexes in the Neurodegenerative Diseases
Toxicology and Environmental Health Sciences, 2019Extracellular exosomes formed inside the cytoplasm of cells are small membranous vesicles that provide an important mode of cell-to-cell communication by delivering proteins, lipids and RNA to target cells. Exosomes are found associated with neurodegenerative diseases mainly Parkinson’s disease (PD) and Alzheimer’s diseases (AD), characterized by ...
Yang-Hoon Kim +6 more
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Crystal structure of an RNA-bound 11-subunit eukaryotic exosome complex
Nature, 2013The exosome is the major 3'-5' RNA-degradation complex in eukaryotes. The ubiquitous core of the yeast exosome (Exo-10) is formed by nine catalytically inert subunits (Exo-9) and a single active RNase, Rrp44. In the nucleus, the Exo-10 core recruits another nuclease, Rrp6.
Debora Lika Makino +2 more
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