Results 41 to 50 of about 315,886 (312)
Purification and analysis of endogenous human RNA exosome complexes [PDF]
RNA, 2016 As a result of its importance in key RNA metabolic processes, the ribonucleolytic RNA exosome complex has been the focus of intense study for almost two decades. Research on exosome subunit assembly, cofactor and substrate interaction, enzymatic catalysis and structure have largely been conducted using complexes produced in the yeast Saccharomyces ...
Michal Domanski +8 more
openaire +4 more sources
The exosome journey: from biogenesis to uptake and intracellular signalling
Cell Communication and Signaling, 2021 The use of exosomes in clinical settings is progressively becoming a reality, as clinical trials testing exosomes for diagnostic and therapeutic applications are generating remarkable interest from the scientific community and investors.
Sonam Gurung +3 more
doaj +1 more source
Flow cytometry with anti-diffraction light sheet (ADLS) by spatial light modulation [PDF]
arXiv, 2023 Flow cytometry is a widespread and powerful technique, whose resolution is determined by its capacity to accurately distinguish fluorescently positive populations from negative ones. However, most informative results are discarded while performing the measurements of conventional flow cytometry, e.g., the cell size, shape, morphology, and distribution ...
arxiv
Mechanical characterization of cells and microspheres sorted by acoustophoresis with in-line resistive pulse sensing [PDF]
Phys. Rev. Applied 13, 034058 (2020), 2019 Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells, expanding the method from size to mechanical sensing could represent a pertinent and innovative tool for cancer ...
arxiv +1 more source
Exosomes in Epilepsy of Tuberous Sclerosis Complex: Carriers of Pro-Inflammatory MicroRNAs [PDF]
Non-Coding RNA, 2021 Exosomes are a class of small, secreted extracellular vesicles (EV) that have recently gained considerable attention for their role in normal cellular function, disease processes and potential as biomarkers. Exosomes serve as intercellular messengers and carry molecular cargo that can alter gene expression and the phenotype of recipient cells. Here, we
Paul M. Stemmer +11 more
openaire +4 more sources
RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis
Nature Communications, 2019 Cytosolic RNA degradation by the RNA exosome requires the Ski complex. Here the authors show that the proteins RST1 and RIPR assist the RNA exosome and the Ski complex in RNA degradation, thereby preventing the production of secondary siRNAs from ...
Heike Lange +15 more
doaj +1 more source
PLoS Genetics, 2020 Accurate target recognition in transcript degradation is crucial for regulation of gene expression. In the fission yeast Schizosaccharomyces pombe, a number of meiotic transcripts are recognized by a YTH-family RNA-binding protein, Mmi1, and selectively ...
Yuichi Shichino +3 more
doaj +1 more source
Ultrasound-based Control of Micro-Bubbles for Exosome Delivery in Treating COVID-19 Lung Damage [PDF]
arXiv, 2021 The recent COVID-19 pandemic has resulted in high fatality rates, especially for patients who suffer from underlying health issues. One of the more serious symptoms exhibited from patients suffering from an acute COVID-19 infection is breathing difficulties and shortness of breath, which is largely due to the excessive fluid (cellular leakage and ...
arxiv
Human Telomerase RNA Processing and Quality Control
Cell Reports, 2015 The non-coding RNA subunit of telomerase provides the template for telomerase activity. In diverse fungi, 3′ end processing of telomerase RNA involves a single cleavage by the spliceosome.
Chi-Kang Tseng +5 more
doaj +1 more source
The RNA Exosome Channeling and Direct Access Conformations Have Distinct In Vivo Functions
Cell Reports, 2016 The RNA exosome is a 3′–5′ ribonuclease complex that is composed of nine core subunits and an essential catalytic subunit, Rrp44. Two distinct conformations of Rrp44 were revealed in previous structural studies, suggesting that Rrp44 may change its ...
Jaeil Han, Ambro van Hoof
doaj +1 more source