From in silico screening to experimental verification: Identification of novel umami peptides from tamarind (<i>Tamarindus indica</i> L.) seed proteins and their validation in enzymatic hydrolysates. [PDF]
Huang S, Li S, Wang X, Jiu X, Cai S.
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Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
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The potential impact of exosomal miR-484 on post-myocardial infarction angiogenesis using bioinformatics and experimental verification. [PDF]
Wu D +6 more
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Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Correction: Chai et al. Exploring Novel Therapeutic Targets in Breast Cancer via Comprehensive Omics Profiling and Experimental Verification. <i>Biology</i> 2025, <i>14</i>, 405. [PDF]
Chai S, Cui J, Sun Y, Wang X, Cai C.
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Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
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Integrated bioinformatic multi-omics analysis and experimental verification reveal the oncogenic role of WDHD1 and its underlying mechanism in gastric cancer progression. [PDF]
Wang X +9 more
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A2M promotes the progression of STAD by upregulating vimentin expression and epithelial-mesenchymal transition: boinformatics analysis and experimental verification. [PDF]
Jiang W +11 more
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A putative prognostic model for lung adenocarcinoma based on crotonylation-related genes by bioinformatics and experimental verification. [PDF]
Wang W +8 more
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Identification and Experimental Verification Reveal SLC2A3 Associated With Prognosis and Immune Infiltration in Colon Adenocarcinoma. [PDF]
Zhao Z, Ju K, Lv J, Lu Z, Luo L.
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