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Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai +8 more
wiley +1 more source
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The International Journal of Artificial Organs, 1980
Artificial eardrums made from biodegradable poly(D, L-lactic acid), poly(glycolic acid) and poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50, and made from the microporous poly(tetrafluoroethylene) and bisphenol-A poly(carbonate) membranes were implanted into the ear and as a reference subcutaneously in rats.
Feenstra, L. +3 more
openaire +3 more sources
Artificial eardrums made from biodegradable poly(D, L-lactic acid), poly(glycolic acid) and poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50, and made from the microporous poly(tetrafluoroethylene) and bisphenol-A poly(carbonate) membranes were implanted into the ear and as a reference subcutaneously in rats.
Feenstra, L. +3 more
openaire +3 more sources
Experimental Economics and Experimental Sociology
2019Abstract Experimental economics has moved beyond the traditional focus on market mechanisms and the “invisible hand” by applying sociological and socio-psychological knowledge in the study of rationality, markets, and efficiency. This knowledge includes social preferences, social norms, and cross-cultural variation in motivations.
GEREKE, Johanna Katharina +1 more
openaire +2 more sources

