Results 1 to 10 of about 3,569,345 (266)
A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter.
Christine Jolicoeur, Michel Cayouette
doaj +5 more sources
Arabidopsis Leaf Explant Culture
In this protocol, Arabidopsis leaf explant culture is described using an adaptation of a previous method (Hu et al., 2000). Cells from the cut edges of leaf explant are able to proliferate and subsequently form calli on the callus induction medium, in ...
Jing-Jing Wang+2 more
doaj +4 more sources
Chick Neural Tube Explant Culture
The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time.
Zhanna Alekseenko+2 more
doaj +4 more sources
Explant Culture of Ovarian Tissue
INTRODUCTION: In this study, it was aimed to isolate, reproduce and characterize stromal cells migrating from tissues by creating ovarian tissue explant culture.
Murat Serkant Ünal+6 more
doaj +2 more sources
Explant culture of human colon
Human colonic epithelium has been cultured as explants in a chemically defined medium for periods of 1 to 20 days. The viability of the explants was shown by the preservation of the ultrastructural features of the colonic epithelial cells and by active incorporation of radioactive precursors into cellular DNA and protein.
Lucy A. Barrett+15 more
openaire +5 more sources
Human Skin Explant Preparation and Culture
The ex vivo experimentation with surgically discarded human skin represents a unique methodology amenable for mechanism and pharmacologic agent studies without the involvement of human subjects.
Jessica Shannon+2 more
doaj +3 more sources
Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy. [PDF]
Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties.
A Dénise den Haan+6 more
doaj +2 more sources
Nucleus pulposus explant culture model [PDF]
AbstractMany of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro
Gang Feng+4 more
openaire +4 more sources
An improved human skin explant culture method for testing and assessing personal care products
Cultured human skin models have been widely used in the evaluation of dermato‐cosmetic products as alternatives to animal testing and expensive clinical testing.
Linli Zhou+8 more
semanticscholar +1 more source
Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation
During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the ...
N. Kupper+4 more
semanticscholar +1 more source