Results 1 to 10 of about 65,983 (248)
A bovine nucleus pulposus explant culture model [PDF]
Journal of Orthopaedic Research, 2021 AbstractLow back pain is a global health problem that is frequently caused by intervertebral disc degeneration (IVDD). Sulfated glycosaminoglycans (sGAGs) give the healthy nucleus pulposus (NP) a high fixed charge density (FCD), which creates an osmotic pressure that enables the disc to withstand high compressive forces.
Salzer, Elias +3 more
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Explant Culture of Ovarian Tissue
Van Medical Journal, 2022 INTRODUCTION: In this study, it was aimed to isolate, reproduce and characterize stromal cells migrating from tissues by creating ovarian tissue explant culture. It is also aimed to create a mixed cell culture (ovarian stromal stem cells and ovarian surface epithelium) with the tissues obtained from different parts of the ovary and to examine the ...
Murat Serkant Ünal +6 more
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Advantages of Adult Mouse Dorsal Root Ganglia Explant Culture in Investigating Myelination in an Inherited Neuropathic Mice Model. [PDF]
Methods Protoc, 2022 A co-culture of neurons and Schwann cells has frequently been used to investigate myelin sheath formation. However, this approach is restricted to myelin-related diseases of the peripheral nervous system.
Mo YJ, Kim YS, Kim MS, Lee YI.
europepmc +2 more sources
Explant culture of human colon
Gastroenterology, 1978 Human colonic epithelium has been cultured as explants in a chemically defined medium for periods of 1 to 20 days. The viability of the explants was shown by the preservation of the ultrastructural features of the colonic epithelial cells and by active incorporation of radioactive precursors into cellular DNA and protein.
Herman Autrup +7 more
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BIO-PROTOCOL, 2014 A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter. Retinal explants can be prepared any time between embryonic day 13 (E13) and postnatal day 4 (P4). Although retinal ganglion cells tend to degenerate shortly after they are generated in explants, and ...
Christine Jolicoeur, Michel Cayouette
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Arabidopsis Leaf Explant Culture
BIO-PROTOCOL, 2015 In this protocol, Arabidopsis leaf explant culture is described using an adaptation of a previous method (Hu et al., 2000). Cells from the cut edges of leaf explant are able to proliferate and subsequently form calli on the callus induction medium, in which is supplemented with 2,4-D and 6-benzyl aminopurine [6-BA].
Lisha Zhang +2 more
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Human Skin Explant Preparation and Culture
BIO-PROTOCOL, 2022 The ex vivo experimentation with surgically discarded human skin represents a unique methodology amenable for mechanism and pharmacologic agent studies without the involvement of human subjects. Here, we describe a protocol that includes preparation, culture, and stimulation of human skin explants, and subsequent analyses by quantitative reverse ...
Shannon, Jessica L +2 more
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Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation. [PDF]
Bio Protoc, 2019 Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response.
Kinoshita-Kawada M +11 more
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Chick Neural Tube Explant Culture
BIO-PROTOCOL, 2015 The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor proliferation and neuronal ...
Zhanna Alekseenko +2 more
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Tumor Tissue Explant Culture of Patient-Derived Xenograft as Potential Prioritization Tool for Targeted Therapy. [PDF]
Front Oncol, 2019 Despite of remarkable progress made in the head and neck cancer (HNC) therapy, the survival rate of this metastatic disease remain low. Tailoring the appropriate therapy to patients is a major challenge and highlights the unmet need to have a good ...
Ghosh S +13 more
europepmc +2 more sources