Bio-Protocol, 2014 A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter.
Christine Jolicoeur, Michel Cayouette
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Adipose tissue explant culture using PDMS flow chambers: an alternative to static explant culture [PDF]
AdipocyteAs obesity rates continue to rise, it is important that we can effectively study adipose tissue to understand its physiological contribution in individuals with obesity.
M Cohen +11 more
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Arabidopsis Leaf Explant Culture
Bio-Protocol, 2015 In this protocol, Arabidopsis leaf explant culture is described using an adaptation of a previous method (Hu et al., 2000). Cells from the cut edges of leaf explant are able to proliferate and subsequently form calli on the callus induction medium, in ...
Jing-Jing Wang +2 more
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Explant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet [PDF]
Regenerative Therapy, 2019 Introduction: Carrier-free autologous mucosal epithelial cell sheets have been clinically utilized as a cell therapy for various epithelial disorders. Fabrication of a transplantable oral mucosal epithelial cell sheet without mouse feeder layers requires
Tsunetaro Morino +4 more
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Ex-Vivo Skin Explant Culture Is a Model for TSLP-Mediated Skin Barrier Immunity. [PDF]
Life (Basel), 2021 The skin is the outermost barrier protecting the body from pathogenic invasion and environmental insults. Its breakdown initiates the start of skin inflammation.
Bauer T +5 more
europepmc +2 more sources
Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation. [PDF]
Int J Mol Sci, 2021 During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the ...
Kupper N +4 more
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Initiation of Supporting Cell Activation for Hair Cell Regeneration in the Avian Auditory Epithelium: An Explant Culture Model. [PDF]
Front Cell Neurosci, 2020 Sensorineural hearing loss is a common disability often caused by the loss of sensory hair cells in the cochlea. Hair cell (HCs) regeneration has long been the main target for the development of novel therapeutics for sensorineural hearing loss.
Matsunaga M +7 more
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Explant Culture of Ovarian Tissue
Van Tıp Dergisi, 2022 INTRODUCTION: In this study, it was aimed to isolate, reproduce and characterize stromal cells migrating from tissues by creating ovarian tissue explant culture.
Murat Serkant Ünal +6 more
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Advantages of Adult Mouse Dorsal Root Ganglia Explant Culture in Investigating Myelination in an Inherited Neuropathic Mice Model [PDF]
Methods and Protocols, 2022 A co-culture of neurons and Schwann cells has frequently been used to investigate myelin sheath formation. However, this approach is restricted to myelin-related diseases of the peripheral nervous system.
Yun Jeong Mo +3 more
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Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy. [PDF]
PLoS ONE, 2013 Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties.
A Dénise den Haan +6 more
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