Isolating human dermal fibroblasts using serial explant culture. [PDF]
Stem Cell Investig, 2019 Background The purpose of this study was to introduce an applicable culture technique to isolate human dermal fibroblasts (HDFs); which could also contribute to research, clinical practices, as well as tissue engineering. Methods Samples from the human
Nejaddehbashi F +6 more
europepmc +2 more sources
Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy. [PDF]
PLoS ONE, 2013 Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties.
A Dénise den Haan +6 more
doaj +2 more sources
FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies. [PDF]
Oxid Med Cell Longev, 2018 Preclinical toxicity screening of the new retinal compounds is an absolute requirement in the pathway of further drug development. Since retinal neuron cultivation and in vivo studies are relatively expensive and time consuming, we aimed to create a fast
Smedowski A +5 more
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Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation [PDF]
Bio-Protocol, 2019 Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response.
Mariko Kinoshita-Kawada +11 more
doaj +2 more sources
FASEB J, 2019 Cellular senescence is a phenotypic state that contributes to age‐related diseases through the secretion of matrix‐degrading and inflammatory molecules. An emerging therapeutic strategy for osteoarthritis (OA) is to selectively eliminate senescent cells ...
Sessions GA +5 more
europepmc +2 more sources
Release of pro-inflammatory cytokines from muscle and bone causes tenocyte death in a novel rotator cuff in vitro explant culture model. [PDF]
Connect Tissue Res, 2018 Purpose: Tendinopathy is a significant clinical problem thought to be associated with altered mechanical loading. Explant culture models allow researchers to alter mechanical loading in a controlled in vitro environment while maintaining tenocytes in ...
Connizzo BK, Grodzinsky AJ.
europepmc +2 more sources
Explant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet [PDF]
Regenerative Therapy, 2019 Introduction: Carrier-free autologous mucosal epithelial cell sheets have been clinically utilized as a cell therapy for various epithelial disorders. Fabrication of a transplantable oral mucosal epithelial cell sheet without mouse feeder layers requires
Tsunetaro Morino +4 more
doaj +2 more sources
Organotypic brain explant culture as a drug evaluation system for malignant brain tumors. [PDF]
Cancer Med, 2017 Therapeutic options for malignant brain tumors are limited, with new drugs being continuously evaluated. Organotypic brain slice culture has been adopted for neuroscience studies as a system that preserves brain architecture, cellular function, and the ...
Minami N +11 more
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Nucleus pulposus explant culture model [PDF]
Journal of Orthopaedic Research, 2009 AbstractMany of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro
G. Feng, Yuqing Wan, F. Shen, Xu-dong Li
semanticscholar +4 more sources
Tumor Tissue Explant Culture of Patient-Derived Xenograft as Potential Prioritization Tool for Targeted Therapy [PDF]
Frontiers in Oncology, 2019 Despite of remarkable progress made in the head and neck cancer (HNC) therapy, the survival rate of this metastatic disease remain low. Tailoring the appropriate therapy to patients is a major challenge and highlights the unmet need to have a good ...
Susmita Ghosh +15 more
doaj +2 more sources