Results 11 to 20 of about 3,692,560 (357)
Human Skin Explant Preparation and Culture
Bio-Protocol, 2022 The ex vivo experimentation with surgically discarded human skin represents a unique methodology amenable for mechanism and pharmacologic agent studies without the involvement of human subjects.
Jessica Shannon +2 more
doaj +3 more sources
Isolating human dermal fibroblasts using serial explant culture. [PDF]
Stem Cell Investig, 2019 Background The purpose of this study was to introduce an applicable culture technique to isolate human dermal fibroblasts (HDFs); which could also contribute to research, clinical practices, as well as tissue engineering. Methods Samples from the human
Nejaddehbashi F +6 more
europepmc +2 more sources
Tyrosine Phosphorylation of p27Kip1 Correlates with Palbociclib Responsiveness in Breast Cancer Tumor Cells Grown in Explant Culture. [PDF]
Mol Cancer Res, 2019 Cdk4-targeting drugs, such as palbociclib, are approved for metastatic ER/PR+, Her2− breast cancer. However, other than loss of retinoblastoma, which is very rare in this subset, there are no biomarkers to predict response.
Gottesman SRS +6 more
europepmc +2 more sources
FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies. [PDF]
Oxid Med Cell Longev, 2018 Preclinical toxicity screening of the new retinal compounds is an absolute requirement in the pathway of further drug development. Since retinal neuron cultivation and in vivo studies are relatively expensive and time consuming, we aimed to create a fast
Smedowski A +5 more
europepmc +2 more sources
Nucleus pulposus explant culture model [PDF]
Journal of Orthopaedic Research, 2009 AbstractMany of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro
Gang, Feng +3 more
openaire +3 more sources
Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation [PDF]
Bio-Protocol, 2019 Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response.
Mariko Kinoshita-Kawada +11 more
doaj +2 more sources
FASEB J, 2019 Cellular senescence is a phenotypic state that contributes to age‐related diseases through the secretion of matrix‐degrading and inflammatory molecules. An emerging therapeutic strategy for osteoarthritis (OA) is to selectively eliminate senescent cells ...
Sessions GA +5 more
europepmc +2 more sources
Release of pro-inflammatory cytokines from muscle and bone causes tenocyte death in a novel rotator cuff in vitro explant culture model. [PDF]
Connect Tissue Res, 2018 Purpose: Tendinopathy is a significant clinical problem thought to be associated with altered mechanical loading. Explant culture models allow researchers to alter mechanical loading in a controlled in vitro environment while maintaining tenocytes in ...
Connizzo BK, Grodzinsky AJ.
europepmc +2 more sources
A bovine nucleus pulposus explant culture model. [PDF]
J Orthop Res, 2022 AbstractLow back pain is a global health problem that is frequently caused by intervertebral disc degeneration (IVDD). Sulfated glycosaminoglycans (sGAGs) give the healthy nucleus pulposus (NP) a high fixed charge density (FCD), which creates an osmotic pressure that enables the disc to withstand high compressive forces.
Salzer E +3 more
europepmc +6 more sources
Organotypic brain explant culture as a drug evaluation system for malignant brain tumors. [PDF]
Cancer Med, 2017 Therapeutic options for malignant brain tumors are limited, with new drugs being continuously evaluated. Organotypic brain slice culture has been adopted for neuroscience studies as a system that preserves brain architecture, cellular function, and the ...
Minami N +11 more
europepmc +2 more sources