Results 251 to 260 of about 66,082 (347)

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis. [PDF]

J Vis Exp, 2017
Lv J, Liu F.
europepmc   +1 more source

Cas9‐ and Cas12a‐mediated excision and replacement of the celiac disease‐related α‐gliadin immunogenic complex in hexaploid wheat

Plant Biotechnology Journal, EarlyView.
Summary Celiac disease (CD) is a chronic enteropathy affecting approximately 1% of the global population. Wheat α‐gliadins are a major contributor to the autoimmune response, as they contain one of the most immunogenic peptides, the 33‐mer, along with numerous variants.
Miriam Marín‐Sanz, Susana Sánchez‐León, María H. Guzmán‐López, Colby G. Starker, Daniel F. Voytas, Francisco Barro   +5 more
wiley   +1 more source

3D Approaches to Culturing Bovine Skin: Explant Culture versus Organotypic Skin Model.

Cells Tissues Organs
Baumbach CM, Anantama NA, Savkovic V, Mülling CKW, Schinköthe J, Michler JK.   +5 more
europepmc   +1 more source

Efficient and heritable gene editing through CRISPR‐Cas9 in Pisum sativum

Plant Biotechnology Journal, EarlyView.
Chloé Soulard, Manon Monfort, Jean‐Paul Pillot, Carine Gery, Laura Chauvin, Stéphanie Morlière, Angélique Lesné, Akiko Sugio, Marie‐Laure Pilet‐Nayel, Catherine Rameau, Fabien Nogué, Alexandre de Saint Germain   +11 more
wiley   +1 more source

Application of a GRF‐GIF chimera enhances plant regeneration for genome editing in tomato

Plant Biotechnology Journal, EarlyView.
Summary Genome editing has become a routine tool for functionally characterizing plant and animal genomes. However, stable genome editing in plants remains limited by the time‐ and labour‐intensive process of generating transgenic plants, as well as by the efficient isolation of desired heritable edits.
Gwen Swinnen, Eléonore Lizé, Miguel Loera Sánchez, Stéphanie Stolz, Sebastian Soyk   +4 more
wiley   +1 more source

Primary explant culture and collagen I substrate enhances corneal endothelial cell morphology. [PDF]

BMC Res Notes, 2018
Zavala J, Montalvo-Parra MD, Guerrero-Ramírez GI, Rodríguez-Barrientos CA, Treviño V, Valdez-García JE.   +5 more
europepmc   +1 more source

A visual monitoring DNA‐free multi‐gene editing system excised via LoxP::FRT/FLP in poplar

Plant Biotechnology Journal, EarlyView.
Summary The CRISPR‐Cas9 system has emerged as a potent molecular scalpel for precise genome editing, and profoundly revolutionized plant genetics and breeding, facilitating the development of innovative and improved plant varieties. Typically, the CRISPR‐Cas9 gene‐editing construct is delivered into target organisms via Agrobacterium tumefaciens ...
Ruqian Wu, Yinghui Chai, Ying Li, Tingting Chen, Wanxin Qi, Yinxuan Xue, Hailin Lu, Zhong Chen, Bin Guo, Shanwen Li, Debin Qin, Xinmin An   +11 more
wiley   +1 more source

Organotypic brain explant culture as a drug evaluation system for malignant brain tumors. [PDF]

Cancer Med, 2017
Minami N, Maeda Y, Shibao S, Arima Y, Ohka F, Kondo Y, Maruyama K, Kusuhara M, Sasayama T, Kohmura E, Saya H, Sampetrean O.   +11 more
europepmc   +1 more source

A pair of GPI‐anchored proteins regulate soybean immunity and disease resistance in coordination with GmLMM1 and GmRALFs

Plant Biotechnology Journal, EarlyView.
Summary Plant cell‐surface‐localized pattern recognition receptors and co‐receptors recognize conserved microbial patterns to initiate pattern‐triggered immunity (PTI), which confers moderate and broad‐spectrum resistance to most pathogens. For instance, the FLS2 receptor recognizes bacterial flagellin (or epitope flg22) by forming a complex with its ...
Weijie Wang, Rubin Chen, Dongmei Wang, Wei Zhang, Qian Zhang, Ye Zhang, Guangyuan Xu, Jun Yang, Zhiyuan Cheng, Suxin Yang, Jinlong Wang, Daolong Dou, Xiangxiu Liang, Xianzhong Feng   +13 more
wiley   +1 more source

Release of pro-inflammatory cytokines from muscle and bone causes tenocyte death in a novel rotator cuff in vitro explant culture model. [PDF]

Connect Tissue Res, 2018
Connizzo BK, Grodzinsky AJ.
europepmc   +1 more source