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The Journal of Physiology, EarlyView.Abstract figure legend Transforming growth factor (TGF)‐β2 and interleukin (IL)‐1β induced morphological and molecular changes consistent with endothelial‐to‐mesenchymal transition (EndMT) in HUVECs isolated from both pregnancies complicated by gestational diabetes (GDM) and non‐GDM pregnancies.
Abigail R. Byford +9 more
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Explant organ culture: A review
Cytotechnology, 1991Organ explant culture models offer several significant advantages for studies of patho-physiologic mechanisms like cell injury, secretion, differentiation and structure development. Organs or small explants/slices can be removed in vivo and maintained in vitro for extended periods of time if careful attention is paid to the media composition, substrate
J H, Resau +4 more
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Organotypic Retinal Explant Cultures from Macaque Monkey
Journal of Visualized Experiments, 2022Hereditary retinal degeneration (RD) is characterized by progressive photoreceptor cell death. Overactivation of the cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG) pathway in photoreceptor cells causes photoreceptor cell death, especially in models harboring phosphodiesterase 6b (PDE6b) mutations.
Wenrong, Xu +5 more
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2014
To investigate chondrocyte biology in an organized structure, limb explant cultures have been established that allow the cultivation of the entire cartilaginous skeletal elements. In these organ cultures, the arrangement of chondrocytes in the cartilage elements and their interaction with the surrounding perichondrium and joint tissue are maintained ...
Wülling, Manuela, Vortkamp, Andrea
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To investigate chondrocyte biology in an organized structure, limb explant cultures have been established that allow the cultivation of the entire cartilaginous skeletal elements. In these organ cultures, the arrangement of chondrocytes in the cartilage elements and their interaction with the surrounding perichondrium and joint tissue are maintained ...
Wülling, Manuela, Vortkamp, Andrea
openaire +2 more sources
Preparation of Retinal Explant Cultures
2023Organotypic retinal explants are routinely used as alternatives to in vitro cell culture and to replace the use of animals in modelling retinal neurodegenerative diseases. Retinal explants fill the gap between in vivo which are expensive, time consuming, and complex due to inaccessibility of target tissues.
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Cytoarchitecture in cultured rat neocortex explants
International Journal of Developmental Neuroscience, 1988AbstractNeocortex explants obtained from 6‐day‐old rat pups and cultured in a serum‐free medium from 5 hr to 13 days in vitro (DIV) show preservation of cytoarchitectural characteristics. Major changes in the size of the explants and their layers occur during the first 2 DIV.
de Jong, B. M. +2 more
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Porcine RPE/Choroidal Explant Cultures
2019The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch's membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings.
Alexa, Klettner, Yoko, Miura
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Explant Cultures of White Adipose Tissue
2008Obesity is characterized by increased adiposity of visceral and subcutaneous depots as well as other organs, including the vasculature. These fat depots secrete various hormone-like proteins implicated in metabolic homeostasis (e.g., adiponectin, resistin), the central control of appetite (e.g., leptin) and the increased production of cytokines.
Sébastien, Thalmann +2 more
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