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Explant Culture for Studying Lung Development
2018Lung development is a complex process that requires the input of various signaling pathways to coordinate the specification and differentiation of multiple cell types. Ex vivo culture of the lung is a very useful technique that represents an attractive model for investigating many different processes critical to lung development, function, and disease ...
Behzad Yeganeh +2 more
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Dopaminergic neurons in explants of substantia nigra in culture
Journal of Neurobiology, 1973AbstractExplants of substantia nigra and corpus striatum obtained from newborn rats were maintained in tissue culture for up to six days. Explants of substantia nigra exhibited a net increase in the ability to take up H3‐dopamine, a process associated with the dopaminergic neurons; in contrast, the explants of corpus striatum showed a rapid loss in ...
Julius Axelrod +3 more
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Culture of Mouse Embryonic Foregut Explants
2014The ability to culture embryonic organ rudiments and follow their development ex vivo has helped to understand how tissues are constructed and what cellular and biological events are important in this process. Here we outline a technique for isolation and ex vivo growth of foregut explants from E8.5 mouse embryos.
Felicia Chen +2 more
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Porcine RPE/Choroidal Explant Cultures
2019The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch's membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings.
Yoko Miura, Alexa Klettner
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Sterilisation of explants and cultures with sodium dichloroisocyanurate
Plant Growth Regulation, 1996The effectiveness of Sodium dichloroisocyanurate as a disinfectant for micropropagated plants was assessed. Analysis of the microbial flora of micropropagated plants showed a wide range of bacteria with predominantly Pseudomonas, Xanthomonas and Actinomycetes.
M. Parkinson +2 more
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Explant Culture of Human and Rabbit Articular Chondrocytes
Connective Tissue Research, 1978Copious outgrowth of chondrocytes was obtained by explantation from each of three rabbit and one surgically-resected human articular cartilages pretreated briefly with trypsin. In lapine explants, ascorbate (40 micrograms/ml) increased DNA three-fold over control values and resulted in deposition of a chondroid matrix.
Charles J. Malemud +2 more
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Cell and explant culture of olfactory chemoreceptor cells
Journal of Neuroscience Methods, 1985An in vitro system for the study of maturation of rat and chick embryonic olfactory receptor cells is presented. A variety of dissociating agents, culture media and substrata were tried in attempts to obtain a preparation of mature living olfactory receptor cells readily visible in the microscope.
Robert C. Gesteland +2 more
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Synchronized neuronal activities in neocortical explant cultures
Experimental Brain Research, 1989Intracellular recordings revealed that in neocortical explant cultures prepared on the day of birth and examined 3-6 weeks later, neurons mature and establish complex synaptic relationships that lead to spontaneous and triggered synchronous discharge. The spontaneous synchronous activity took several forms, including periodic generation of epileptiform
B. Wolfson +2 more
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Chapter 14 Explant Methods for Epidermal Cell Culture
1980Publisher Summary This chapter discusses the explant methods for epidermal cell culture. Full-thickness human skin is composed of epidermis, dermis, and subcutaneous tissue. Dermal components such as fibroblasts have been useful in studies on somatic cell genetics, cellular aging, and transformation.
Susan M. Fischer +3 more
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Effects of hyperthermia on cultured explants of porcine endometrium
British Veterinary Journal, 1986Abstract Explants of endometrium were cultured in medium M199 plus 10% sow's serum, and in a gas phase of 5% CO2 in air, for up to 13 days at temperatures of either 38 °C or 41 °C. Assessed by gross and microscopical appearance, survival of the explants was significantly worse at 41 °C.
M. Trujano, A.E. Wrathall
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