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Explant Culture for Studying Lung Development
2018Lung development is a complex process that requires the input of various signaling pathways to coordinate the specification and differentiation of multiple cell types. Ex vivo culture of the lung is a very useful technique that represents an attractive model for investigating many different processes critical to lung development, function, and disease ...
Behzad, Yeganeh +2 more
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Synchronized neuronal activities in neocortical explant cultures
Experimental Brain Research, 1989Intracellular recordings revealed that in neocortical explant cultures prepared on the day of birth and examined 3-6 weeks later, neurons mature and establish complex synaptic relationships that lead to spontaneous and triggered synchronous discharge. The spontaneous synchronous activity took several forms, including periodic generation of epileptiform
M J, Gutnick, B, Wolfson, F, Baldino
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Culture of Mouse Embryonic Foregut Explants
2014The ability to culture embryonic organ rudiments and follow their development ex vivo has helped to understand how tissues are constructed and what cellular and biological events are important in this process. Here we outline a technique for isolation and ex vivo growth of foregut explants from E8.5 mouse embryos.
Felicia, Chen, Wellington V, Cardoso
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Explant culture of human fetal small intestine
Gastroenterology, 1985Human fetal intestine (10-14 wk gestation) has been cultured as explants in a serum-free Leibovitz L-15 medium for periods up to 9 days. As determined by light microscopy, the overall architecture of the intestinal explant was maintained throughout the culture period.
D, Ménard, P, Arsenault
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Improving Endothelial Explant Tissue Culture by Novel Thermoresponsive Cell Culture System
Current Eye Research, 2020Studying cell migration of corneal endothelial cells in vitro is challenging because the capacity for cell migration needs to be maintained while at the same time the tissue must remain fixed on a rigid substrate. In this study, we report a thermoresponsive culture technique designed to maintain cellular viability, and to reduce tissue handling in ...
Miron, A. +5 more
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Cleavage Blastomere Explant Culture in Xenopus
Cold Spring Harbor Protocols, 2018The individual blastomeres of Xenopus two- to 32-cell embryos have been fate mapped. This work identified the precursors of most of the embryonic cell types, tissues and organs; however, the maps do not reveal the cell interactions or signaling pathways that are required for establishing cell fates.
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Murine Limb Explant Cultures to Assess Cartilage Development
2020To investigate chondrocyte biology in an organized structure, limb explant cultures have been established that allow for the cultivation of the entire cartilaginous skeletal elements. In these organ cultures, the arrangement of chondrocytes in the cartilage elements and their interaction with the surrounding perichondrium and joint tissue are ...
Wülling, Manuela, Vortkamp, Andrea
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Cell Cultures from Tissue Explants
1974Cell cultures can be prepared from the most diverse tissues and organs. The basic procedure is as follows: Tissue specimens (biopsy or autopsy material) are taken from the body, reduced to small pieces, and set up in vitro in a nutrient medium. There are a number of methods available to prevent expiant fragments from floating in the nutrient medium. In
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Manipulation of Shoot Formation in Cultured Explants
2003Regeneration of buds, shoots, and roots in cells and explants in vitro has provided useful developmental systems to analyze the processes of cell differentiation and morphogenesis. Interest in these studies was greatly stimulated by the demonstration of chemical regulation of morphogenesis in 1957 by Skoog and Miller (1).
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Neural Explant Cultures from Xenopus laevis.
Journal of visualized experiments : JoVE, 2013The complex process of axon guidance is largely driven by the growth cone, which is the dynamic motile structure at the tip of the growing axon. During axon outgrowth, the growth cone must integrate multiple sources of guidance cue information to modulate its cytoskeleton in order to propel the growth cone forward and accurately navigate to find its ...
Laura Anne, Lowery +3 more
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