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Preparation of Retinal Explant Cultures
Methods in molecular biology, 2023Organotypic retinal explants are routinely used as alternatives to in vitro cell culture and to replace the use of animals in modelling retinal neurodegenerative diseases. Retinal explants fill the gap between in vivo which are expensive, time consuming, and complex due to inaccessibility of target tissues.
Zubair Ahmed
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Explant Culture for Studying Lung Development
Methods in molecular biology, 2018Lung development is a complex process that requires the input of various signaling pathways to coordinate the specification and differentiation of multiple cell types. Ex vivo culture of the lung is a very useful technique that represents an attractive model for investigating many different processes critical to lung development, function, and disease ...
Behzad, Yeganeh +2 more
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A Novel Method for Coral Explant Culture and Micropropagation
Marine Biotechnology, 2010We describe here a method for the micropropagation of coral that creates progeny from tissue explants derived from a single polyp or colonial corals. Coral tissue explants of various sizes (0.5-2.5 mm in diameter) were manually microdissected from the solitary coral Fungia granulosa.
Maya, Vizel +3 more
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Explant organ culture: A review
Cytotechnology, 1991Organ explant culture models offer several significant advantages for studies of patho-physiologic mechanisms like cell injury, secretion, differentiation and structure development. Organs or small explants/slices can be removed in vivo and maintained in vitro for extended periods of time if careful attention is paid to the media composition, substrate
J H, Resau +4 more
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Long term explant culture for harvesting homogeneous population of human dental pulp stem cells
Cell Biology International, 2018Dental pulp stem cells have emerged as a preferred source of mesenchymal stem cells, because of its easy availability and high stem cell content.
Vikrant R Patil +4 more
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Cytoarchitecture in cultured rat neocortex explants
International Journal of Developmental Neuroscience, 1988AbstractNeocortex explants obtained from 6‐day‐old rat pups and cultured in a serum‐free medium from 5 hr to 13 days in vitro (DIV) show preservation of cytoarchitectural characteristics. Major changes in the size of the explants and their layers occur during the first 2 DIV.
de Jong, B. M. +2 more
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2014
To investigate chondrocyte biology in an organized structure, limb explant cultures have been established that allow the cultivation of the entire cartilaginous skeletal elements. In these organ cultures, the arrangement of chondrocytes in the cartilage elements and their interaction with the surrounding perichondrium and joint tissue are maintained ...
Wülling, Manuela, Vortkamp, Andrea
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To investigate chondrocyte biology in an organized structure, limb explant cultures have been established that allow the cultivation of the entire cartilaginous skeletal elements. In these organ cultures, the arrangement of chondrocytes in the cartilage elements and their interaction with the surrounding perichondrium and joint tissue are maintained ...
Wülling, Manuela, Vortkamp, Andrea
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Retinal explant culture: A platform to investigate human neuro‐retina
Clinical and Experimental Ophthalmology, 2018The retina is the tissue responsible for light detection, in which retinal neurons convert light energy into electrical signals to be transported towards the visual cortex.
Aparna P. Murali +4 more
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Explant Cultures of White Adipose Tissue
2008Obesity is characterized by increased adiposity of visceral and subcutaneous depots as well as other organs, including the vasculature. These fat depots secrete various hormone-like proteins implicated in metabolic homeostasis (e.g., adiponectin, resistin), the central control of appetite (e.g., leptin) and the increased production of cytokines.
Sébastien, Thalmann +2 more
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Porcine RPE/Choroidal Explant Cultures
2019The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch's membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings.
Alexa, Klettner, Yoko, Miura
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