Results 321 to 330 of about 3,692,560 (357)
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Explant Culture of Human and Rabbit Articular Chondrocytes
Connective Tissue Research, 1978Copious outgrowth of chondrocytes was obtained by explantation from each of three rabbit and one surgically-resected human articular cartilages pretreated briefly with trypsin. In lapine explants, ascorbate (40 micrograms/ml) increased DNA three-fold over control values and resulted in deposition of a chondroid matrix.
C J, Malemud, D P, Norby, L, Sokoloff
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Culture of Mouse Embryonic Foregut Explants
2014The ability to culture embryonic organ rudiments and follow their development ex vivo has helped to understand how tissues are constructed and what cellular and biological events are important in this process. Here we outline a technique for isolation and ex vivo growth of foregut explants from E8.5 mouse embryos.
Felicia, Chen, Wellington V, Cardoso
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Dopaminergic neurons in explants of substantia nigra in culture
Journal of Neurobiology, 1973AbstractExplants of substantia nigra and corpus striatum obtained from newborn rats were maintained in tissue culture for up to six days. Explants of substantia nigra exhibited a net increase in the ability to take up H3‐dopamine, a process associated with the dopaminergic neurons; in contrast, the explants of corpus striatum showed a rapid loss in ...
J T, Coyle +3 more
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Cell and explant culture of olfactory chemoreceptor cells
Journal of Neuroscience Methods, 1985An in vitro system for the study of maturation of rat and chick embryonic olfactory receptor cells is presented. A variety of dissociating agents, culture media and substrata were tried in attempts to obtain a preparation of mature living olfactory receptor cells readily visible in the microscope.
F, Gonzales +2 more
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Synchronized neuronal activities in neocortical explant cultures
Experimental Brain Research, 1989Intracellular recordings revealed that in neocortical explant cultures prepared on the day of birth and examined 3-6 weeks later, neurons mature and establish complex synaptic relationships that lead to spontaneous and triggered synchronous discharge. The spontaneous synchronous activity took several forms, including periodic generation of epileptiform
M J, Gutnick, B, Wolfson, F, Baldino
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Effects of hyperthermia on cultured explants of porcine endometrium
British Veterinary Journal, 1986Abstract Explants of endometrium were cultured in medium M199 plus 10% sow's serum, and in a gas phase of 5% CO2 in air, for up to 13 days at temperatures of either 38 °C or 41 °C. Assessed by gross and microscopical appearance, survival of the explants was significantly worse at 41 °C.
M, Trujano, A E, Wrathall
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In vitro culture of equine respiratory mucosa explants
The Veterinary Journal, 2009An in vitro model of the upper respiratory tract of the horse was developed to investigate mechanisms of respiratory diseases. Four tissues of the upper respiratory tract of three horses were collected. Explants were maintained in culture at an air-liquid interface for 96h.
Annelies, Vandekerckhove +5 more
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Ciliary epithelia of the mammalian eye in cultured explants
Cell and Tissue Research, 1983The ultrastructural characteristics of ciliary epithelium from bovine, pigmented rabbit, and fetal albino rabbit were studied in cultured explants. The tips of ciliary processes were cultured in plastic dishes with Dulbecco Modified Eagle Medium (DMEM) containing 5% fetal bovine serum. More than half of the explants adhered to the plastic culture dish,
K, Kondo, M, Coca-Prados, M, Sears
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Culturing spinal cord explants in a collagen gel
Journal of Neuroscience Methods, 1984A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen.
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Chemosensitivity of medullary neurons in explant tissue cultures
Neuroscience, 1991To determine whether cultured medulla contains chemosensitive neurons which are excited by CO2 and fixed acid and whether this function is specific to the ventral medulla, tissue explants of ventral and dorsal medulla were prepared from neonatal rats and incubated for two to three weeks.
J A, Neubauer +4 more
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