Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey +4 more
wiley +1 more source
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source
New Conditions for the Exponential Stability of Pseudolinear Difference Equations in Banach Spaces
We study the local exponential stability of evolution difference systems with slowly varying coefficients and nonlinear perturbations. We establish the robustness of the exponential stability in infinite-dimensional Banach spaces, in the sense that the ...
Rigoberto Medina
doaj +1 more source
Exponential stability for linear evolutionary equations [PDF]
We give an approach to exponential stability within the framework of evolutionary equations due to Picard [Math. Methods Appl. Sci. 32(14) (2009), 1768–1803]. We derive sufficient conditions for exponential stability in terms of the material law operator which is defined via an analytic and bounded operator-valued function and give an estimate for the ...
openaire +2 more sources
Super-stability and stability of the exponential equations [PDF]
In this paper, we introduce the concepts of super-stability, stability, δ -Ger-stability of the exponential equation of an operator from a normed space into a normed algebra. By using some results due to J. Baker and R. Ger, we obtain some new conclusions about these stabilities. Mathematics subject classification (2010): Primary 39B52, secondary 39B82,
Lu Xu +3 more
openaire +1 more source
MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss +7 more
wiley +1 more source
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Exponential Stability of Switched Positive Homogeneous Systems
This paper studies the exponential stability of switched positive nonlinear systems defined by cooperative and homogeneous vector fields. In order to capture the decay rate of such systems, we first consider the subsystems.
Dadong Tian, Shutang Liu
doaj +1 more source
A Tutorial on Incremental Stability Analysis using Contraction Theory [PDF]
This paper introduces a methodology for differential nonlinear stability analysis using contraction theory (Lohmiller and Slotine, 1998). The methodology includes four distinct steps: the descriptions of two systems to be compared (the plant and the ...
J. Jouffroy, T.I. Fossen
doaj +1 more source

