Intragenic tandem repeats in Daphnia magna: structure, function and distribution [PDF]
Background Expressed sequence tag (EST) databases provide a valuable source of genetic data in organisms whose genome sequence information is not yet compiled.
Ebert, Dieter +8 more
core +1 more source
Long non-coding RNAs in wheat are related to its susceptibility to powdery mildew
To identify genes involved in the susceptibility to powdery mildew in wheat, genechip hybridization experiments were performed applying interactions between resistant and susceptible wheat cultivars and powdery mildew.
S. H. Li +5 more
doaj +1 more source
Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites [PDF]
Parasitism is a highly successful mode of life and one that requires suites of gene adaptations to permit survival within a potentially hostile host.
Murray E Selkirk +20 more
core +1 more source
An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences. [PDF]
BACKGROUND: cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge.
Jacob Pollier +4 more
doaj +1 more source
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P. +68 more
core +1 more source
Mining expressed sequence tags identifies cancer markers of clinical interest
Background Gene expression data are a rich source of information about the transcriptional dis-regulation of genes in cancer. Genes that display differential regulation in cancer are a subtype of cancer biomarkers.
Skrabanek Lucy, Campagne Fabien
doaj +1 more source
Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti +14 more
core +1 more source
Comparative Genomics in Switchgrass Using 61,585 High-Quality Expressed Sequence Tags
The development of genomic resources for switchgrass ( L.), a perennial NAD-malic enzyme type C grass, is required to enable molecular breeding and biotechnological approaches for improving its value as a forage and bioenergy crop. Expressed sequence tag
Christian M. Tobias +9 more
doaj +1 more source
Development and validation of new SSR markers from expressed regions in the garlic genome
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems.
Meryem Ipek +4 more
doaj +1 more source
200 000 nematode expressed sequence tags on the Net
Expressed sequence tags (ESTs) are single-pass sequence reads made from randomly selected cDNA clones, which represent the expressed genes of an organism.
Whitton, C. +4 more
core +1 more source

