Analyses of Expressed Sequence Tags from Apple [PDF]
Abstract The domestic apple (Malus domestica; also known as Malus pumila Mill.) has become a model fruit crop in which to study commercial traits such as disease and pest resistance, grafting, and flavor and health compound biosynthesis. To speed the discovery of genes involved in these traits, develop markers to map genes, and breed new
Richard D, Newcomb +17 more
openaire +2 more sources
Update of the Gene Discovery Program in Schistosoma mansoni with the Expressed Sequence Tag Approach
Continuing the Schistosoma mansoni Genome Project 363 new templates were sequenced generating 205 more ESTs corresponding to 91 genes. Seventy four of these genes (81%) had not previously been described in S. mansoni.
Élida ML Rabelo +8 more
doaj +1 more source
An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences. [PDF]
BACKGROUND: cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge.
Jacob Pollier +4 more
doaj +1 more source
Identification of a Novel 81-kDa Component of the Xenopus Origin Recognition Complex [PDF]
The Xenopus origin recognition complex is essential for chromosomal DNA replication in cell-free extracts. We have immunopurified the Xenopus origin recognition complex with anti-Xorc2 antibodies and analyzed its composition and properties.
Carpenter, Phillip B. +1 more
core +1 more source
Expressed sequence tags analysis of Blattella germanica [PDF]
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences ...
Hyang Suk, Chung +7 more
openaire +2 more sources
BackgroundChickpea (Cicer arietinum L.) is an important grain-legume crop that is mainly grown in rainfed areas, where terminal drought is a major constraint to its productivity.
A. Deokar +8 more
semanticscholar +1 more source
Mining expressed sequence tags identifies cancer markers of clinical interest
Background Gene expression data are a rich source of information about the transcriptional dis-regulation of genes in cancer. Genes that display differential regulation in cancer are a subtype of cancer biomarkers.
Skrabanek Lucy, Campagne Fabien
doaj +1 more source
Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers [PDF]
Background Expressed Sequence Tag (EST) has been a cost-effective tool in molecular biology and represents an abundant valuable resource for genome annotation, gene expression, and comparative genomics in plants.
Xiaoying Li +6 more
core +1 more source
Comparative Genomics in Switchgrass Using 61,585 High-Quality Expressed Sequence Tags
The development of genomic resources for switchgrass ( L.), a perennial NAD-malic enzyme type C grass, is required to enable molecular breeding and biotechnological approaches for improving its value as a forage and bioenergy crop. Expressed sequence tag
Christian M. Tobias +9 more
doaj +1 more source
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags [PDF]
Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would result in an expressed sequence tag (EST) database ...
Dias Neto, Emmanuel +23 more
openaire +3 more sources

