Results 1 to 10 of about 506,971 (266)
F1000Research, 2014 Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is.
Vikash Bhardwaj, Kulbhushan Sharma
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Scientific Reports, 2023 The tetrameric tumor suppressor p53 represents a great challenge for 3D-structural analysis due to its high degree of intrinsic disorder (ca. 40%). We aim to shed light on the structural and functional roles of p53’s C-terminal region in full-length ...
Alessio Di Ianni +8 more
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Complementary recognition in condensed DNA: Accelerated DNA renaturation [PDF]
Journal of Molecular Biology, 1991 The functional consequences of DNA condensation are investigated. The recognition of complementary strands is profoundly modified by this critical phenomenon. (1) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including ...
J L, Sikorav, G M, Church
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RNA-programmed genome editing in human cells
eLife, 2013 Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek +5 more
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Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali. [PDF]
PLoS ONE, 2011 Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis.
Takeshi Terahara +7 more
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iScience, 2022 Summary: The DNA origami technique is used to construct custom-shaped nanostructures that can be used as components of two-dimensional crystalline structures with user-defined structural patterns.
Yuki Suzuki +3 more
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Simplified detection of the hybridized DNA using a graphene field effect transistor
Science and Technology of Advanced Materials, 2017 Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA).
Arun Kumar Manoharan +3 more
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Sensors, 2014 A DNA biosensor for detection of specific oligonucleotides sequences of Plum Pox Virus (PPV) in plant extracts and buffer is proposed. The working principles of a genosensor are based on the ion-channel mechanism. The NH2-ssDNA probe was deposited onto a
Kamila Malecka +3 more
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Sensors, 2013 A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution ...
Chee-Seng Toh, Jiajia Deng
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Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA
Molecules, 2022 The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard +3 more
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