Results 1 to 10 of about 1,333,192 (304)
Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
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BioTechniques, 2018 Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli.
Bettina Rohweder +3 more
doaj +1 more source
CRISPR/Cas9 high-throughput screening in cancer research [PDF]
E3S Web of Conferences, 2020 In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely ...
Liu Zhuoxin
doaj +1 more source
Adventures in the enormous: a 1.8 million clone BAC library for the 21.7 Gb genome of loblolly pine. [PDF]
PLoS ONE, 2011 Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to ...
Zenaida V Magbanua +8 more
doaj +1 more source
Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II) [PDF]
, 1993 A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized.
Abelson +28 more
core +1 more source
A Nonpolymorphic Class I Gene in the Murine Major Histocompatibility Complex [PDF]
, 1984 DNA sequence analysis of a class I gene (QlO), which maps to the Qa2,3 locus in the C57BL/lO (H- 2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library.
Flavell, Richard A. +4 more
core +1 more source
htsint: a Python library for sequencing pipelines that combines data through gene set generation [PDF]
, 2015 Background: Sequencing technologies provide a wealth of details in terms of genes, expression, splice variants, polymorphisms, and other features. A standard for sequencing analysis pipelines is to put genomic or transcriptomic features into a context of
Bonneaud, Camille +2 more
core +3 more sources
Analysis of shuffled gene libraries
Journal of Molecular Biology, 2002 In vitro recombination of homologous genes (family shuffling) has been proposed as an effective search strategy for laboratory evolution of genes and proteins. Few data are available, however, on the composition of shuffled gene libraries, from which one could assess the efficiency of recombination and optimize protocols.
Joern, John M +2 more
openaire +3 more sources
Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries
Beilstein Journal of Organic Chemistry, 2018 The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library.
Ruth Suchsland +2 more
doaj +1 more source
Synthetic and Systems Biotechnology, 2017 Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering.
Erqing Jin +5 more
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