Generation and characterization of expressed sequence tags in young roots of tea (Camellia assamica)
Biologia Plantarum, 2016 Assam tea (Camellia assamica) is perennial crop susceptible to moisture stress. We used its tender roots to construct a cDNA library for the identification, functional annotation, and analysis of transcripts. A total of 811 full-length expressed sequence
A. Das, M. Mukhopadhyay, T. K. Mondal
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Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells [PDF]
Jichu yixue yu linchuang, 2021 Objective To establish an inducible CRISPR library screening method for human embryonic stem cells (hESCs) to screen the cis-acting elements involed in the maintainance the pluripotency of hESCs.
SUN Meng-yao, LIU Si-qi, ZHOU Fan-qi, MA Yan-ni, YU Jia
doaj
Microbial population changes during bioremediation of nitroaromatic - and nitramine-contaminated lagoon [PDF]
, 2008 Nitration reactions of aromatic compounds are commonly involved in military industrial processes. Military industries treated their process effluents using lagoon systems for many years.
Goñi, Marisol +5 more
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Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Non-parametric Bayesian modelling of digital gene expression data [PDF]
, 2013 Next-generation sequencing technologies provide a revolutionary tool for generating gene expression data. Starting with a fixed RNA sample, they construct a library of millions of differentially abundant short sequence tags or "reads", which constitute a
Gough, Julian, Vavoulis, Dimitrios V.
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Molecular Oncology, EarlyView.Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra +10 more
wiley +1 more source
MicrobiologyOpen, 2017 Aromatic hydrocarbons (AH), such as polycyclic aromatic hydrocarbons, are compounds largely found in nature. Aromatic‐ring‐hydroxylating dioxygenases (ARHD) are proteins involved in AH degradation pathways.
Sanderson T. P. deSousa +3 more
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Frontiers in Microbiology, 2012 Sphagnum mosses represent a main component in ombrotrophic wetlands. They harbor a specific and diverse microbial community with essential functions for the host. To understand extend and degree of host specificity, Sphagnum fallax and S.
Anastasia eBragina +6 more
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Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, 2020 Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu +6 more
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Cloning and characterisation of two subtilisin-like protease genes from Neotyphodium lolii : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Genetics at Massey University [PDF]
, 2000 PCR amplification of Neotyphodium lolii genomic DNA with degenerate primers detected two different sequences with homology to subtilisin-like proteases. These two PCR products were used to screen a N. lolii Lp19 genomic library.
McGill, Michelle Kay
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