Results 31 to 40 of about 27,029,195 (311)

RiPP recognition elements evolved to prevent pathway interference through leader peptide discrimination

Nature Communications
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are natural products with diverse structures and functions. Here, we report the discovery of a family of RiPPs whose biosynthetic gene clusters are widespread in the Bacillota ...
Aleksandr Popov, Dmitry Bikmetov, Anastasiia Grigoreva, Marina Serebryakova, Konstantin Severinov, Yuri I. Wolf, Guy Lippens, Akira Wada, Shunsuke Tagami, Svetlana Dubiley   +9 more
doaj   +1 more source

Gene expression profile in white alpaca (Vicugna pacos) skin

Animal, 2011
A cDNA library from white alpaca (Vicugna pacos) skin was constructed using SMART technology to investigate the global gene expression profile in alpaca skin and identify genes associated with physiology of alpaca skin and pigmentation.
R. Fan, Y. Dong, J. Cao, R. Bai, Z. Zhu, P. Li, J. Zhang, X. He, L. Lü, J. Yao, M. Mondal, G.W. Smith, C. Dong   +12 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system

Frontiers in Microbiology
Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered
Shijie Hu, Shijie Hu, Mailin Gan, Mailin Gan, Ziang Wei, Ziang Wei, Pan Shang, Pan Shang, Lei Song, Lei Song, Jinkang Feng, Jinkang Feng, Lei Chen, Lei Chen, Lili Niu, Lili Niu, Yan Wang, Yan Wang, Yan Wang, Shunhua Zhang, Shunhua Zhang, Linyuan Shen, Linyuan Shen, Linyuan Shen, Li Zhu, Li Zhu, Li Zhu, Ye Zhao, Ye Zhao, Ye Zhao   +29 more
doaj   +1 more source

Selection and characterization of specific nanobody against bovine virus diarrhea virus (BVDV) E2 protein.

PLoS ONE, 2017
Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into
Tiansen Li, Meiling Huang, Hongran Xiao, Guoqi Zhang, Jinhua Ding, Peng Wu, Hui Zhang, Jinliang Sheng, Chuangfu Chen   +8 more
doaj   +1 more source

Transposase-assisted tagmentation of RNA/DNA hybrid duplexes

eLife, 2020
Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li, Chengqi Yi   +6 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Massive integration of large gene libraries in the chromosome of Escherichia coli

Microbial Biotechnology
Large gene libraries are frequently created in Escherichia coli plasmids, which can induce cell toxicity and expression instability due to the high gene dosage.
Lidia Cerdán, Beatriz Álvarez, Luis Ángel Fernández   +2 more
doaj   +1 more source

Establishment of a CRISPR-Based Lentiviral Activation Library for Transcription Factor Screening in Porcine Cells

Animals
Transcription factors play important roles in the growth and development of various tissues in pigs, such as muscle, fat, and bone. A transcription-factor-scale activation library based on the clustered, regularly interspaced, short palindromic repeat ...
Yingjuan Liang, Xiaoxia Yao, Jingxin Han, Jinpeng Wang, Xiao Zhang, Donglin Zhao, Chaoqian Jiang, Lishuang Geng, Shihao Lv, Zhonghua Liu, Yanshuang Mu   +10 more
doaj   +1 more source

Construction of a combinatorial library of chimeric tumor-specific promoters

BioTechniques, 2017
Gene therapy is a fast-developing field of molecular medicine. New, effective, and cancer-specific promoters are in high demand by researchers seeking to treat cancer through expression of therapeutic genes.
Kirill N. Kashkin, Igor P. Chernov, Dmitry A. Didych, Eugene D. Sverdlov   +3 more
doaj   +1 more source