Solid Phase Assembly of Fully Protected Trinucleotide Building Blocks for Codon-Based Gene Synthesis
Applied Sciences, 2019 The use of pre-formed trinucleotides, representing codons of the 20 canonical amino acids, for oligonucleotide-directed mutagenesis offers the advantage of controlled randomization and generation of “smart libraries”. We here present a method
Ruth Suchsland +3 more
doaj +1 more source
cDNA Cloning Demonstrates the Expression of Pregnancy-Specific Glycoprotein Genes, a Subgroup of the Carcinoembryonic Antigen Gene Family, in Fetal Liver [PDF]
, 1989 The pregnancy-specific glycoprotein (PSG) genes constitute a subgroup of the carcinoembryonic antigen (CEA) gene family. Here we report the cloning of four cDNAs coding for different members of the PSG family from a human fetal liver cDNA library.
Thompson, John A. +2 more
core +1 more source
Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli [PDF]
, 2007 L-Arabinose isomerase (E.C. 5.3.1.14) catalyzes the reversible isomerization between L-arabinose and L-ribulose and is highly selective towards L-arabinose.
Beauprez, Joeri +7 more
core +1 more source
Mutant non-coding RNA resource in mouse embryonic stem cells
Disease Models & Mechanisms, 2021 Gene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells. It is performed with generic gene trap vectors that simultaneously mutate and report the expression of ...
Jens Hansen +2 more
doaj +1 more source
An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]
, 2018 The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J +20 more
core +1 more source
ERgene: Python library for screening endogenous reference genes [PDF]
Scientific Reports, 2020 AbstractIn gene expression analysis, sample differences and experimental operation differences are common, but sometimes, these differences will cause serious errors to the results or even make the results meaningless. Finding suitable internal reference genes efficiently to eliminate errors is a challenge.
Zeng, Zehua +3 more
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Cloning of a Transglutaminase Gene from an Indonesian Streptomyces Strain
Squalen, 2016 Microbial Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer of acyl group. Many microbial strains that produce MTGase belong to Streptomyces members. This research was aimed at cloning of a MTGase gene.
Seprianto Chaniago
doaj +1 more source
Screening technology of cyclic peptide library based on gene encoding
Medicine in Drug Discovery, 2022 As a powerful tool for the discovery of peptide compounds, high-throughput library screening technology can also perform multitarget screening with high chemical diversity as a condition, which accelerates the process of exploring high-affinity peptide ...
Yangqiang Chen +5 more
doaj +1 more source
The COVID-19 Gene and Drug Set Library [PDF]
SSRN Electronic Journal, 2020 Abstract The coronavirus (CoV) severe acute respiratory syndrome (SARS)-CoV-2 (COVID-19) pandemic has received rapid response by the research community to offer suggestions for repurposing of approved drugs as well as to improve our understanding of the COVID-19 viral life cycle molecular mechanisms.
Maxim V. Kuleshov +9 more
openaire +3 more sources
Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells [PDF]
, 1985 cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+
Lazarides, Elias +3 more
core +3 more sources