Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5 [PDF]
BMC Research Notes, 2019 Objective Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa).
Karthikeyan Dharmaraj +3 more
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Production of
BMC Biotechnology, 2007 Background Many structural biology- and high-throughput laboratories experience the acquisition of multiple cDNAs from different sources as a rather time- and resource-consuming procedure. The techniques presented here solve these problems.
Steinmetz Michel O +3 more
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In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. [PDF]
PLoS ONE, 2012 The cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 5'-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are ...
Sangkyou Lee +4 more
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Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein [PDF]
Virology Journal, 2005 Background To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV).
Chen Wei, Lwa Siew
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Construction and identification of yeast two-hybrid cDNA library of Haemonchus contortus
浙江大学学报. 农业与生命科学版, 2022 This experiment aimed to construct a cDNA library of Haemonchus contortus, which could provide the basis for further research on the protein interaction mechanism and screening out the interactive proteins of H. contortus.
ZHANG Hui +8 more
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An improved shotgun antisense method for mutagenesis and gene identification
BioTechniques, 2020 Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations.
Yu Tang, Richard H Gomer
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Tissue-specific Expression of Distinct Spectrin and Ankyrin Transcripts in Erythroid and Nonerythroid Cells [PDF]
, 1985 cDNA probes for three components of the erythroid membrane skeleton, α spectrin, β spectrin, and ankyrin, were obtained by using monospecific antibodies to screen a λgt11 expression vector library containing cDNA prepared from chicken erythroid poly(A)^+
Lazarides, Elias +3 more
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Preparation of cDNA Library for dRNA-seq
Bio-Protocol, 2012 microRNAs (miRNAs) are ubiquitous regulators of gene expression in eukaryotic organisms, which guide Argonaute proteins (AGO) to cleave target mRNA or inhibit its translation based on sequence complementarity. In plants, miRNA directed cleavage occurs on
Feng Li, Barbara Baker
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A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography [PDF]
Journal of Cell and Molecular Research, 2020 We have developed a rapid, quantitative method for analysing the outcome of the first strand synthesis step in cDNA library preparation, yield and molecular weight range of the final cDNA products are determined after size fractionation.
Maryam M. Matin, David P. Hornby
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Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2018 Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in
Nehir OZDEMIR OZGENTURK +9 more
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