Results 21 to 30 of about 420,567 (309)

Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism. [PDF]

PLoS ONE, 2013
Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression ...
Abid Hussain, Yi-Feng Li, Yu Cheng, Yang Liu, Chuan-Cheng Chen, Shuo-Yang Wen   +5 more
doaj   +1 more source

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

Animal, 2013
A buffalo oocyte-specific subtracted cDNA library was constructed to identify exclusively or preferentially oocyte-expressed genes. The library represented an enriched population of transcripts obtained from oocytes of diverse ovarian follicular origin ...
S.K. Rajput, P. Kumar, B. Roy, A. Verma, H.P. Pandey, D. Singh, S. De, T.K. Datta   +7 more
doaj   +1 more source

Human complement factor H [PDF]

, 1986
We isolated cDNA clones coding for the functionally important tryptic N-terminal38- kDa fragment of human complement control protein factor H using polyclonal and monoclonal antibodies to screen a human liver cDNA library cloned in a bacterial expression
Dierich, Manfred P., Schulz, T. F., Schwaeble, W., Stanley, K. K., Weiss, Elisabeth H.   +4 more
core   +1 more source

A Comprehensive Arabidopsis Yeast Two-Hybrid Library for Protein-Protein Interaction Studies: A Resource to the Plant Research Community

Molecular Plant-Microbe Interactions, 2018
Yeast-two-hybrid (Y2H) cDNA library screening is a valuable tool to uncover protein-protein interactions and represents a widely used method to investigate protein function. However, low transcript representation in cDNA libraries limits the depth of the
Cleverson Carlos Matiolli, Maeli Melotto
doaj   +1 more source

Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes for Functional Genomics

BioTechniques, 2001
Here, we describe the application of a RecA-based cloning technology to generate full-length cDNA libraries enriched for genes that are differentially expressed between tumor and normal tissue samples.
B. Zhumabayeva, C. Chang, J. McKinley, L. Diatchenko, P.D. Siebert   +4 more
doaj   +1 more source

Tobacco Genes Induced by the Bacterial Effector Protein AvrPto

Molecular Plant-Microbe Interactions, 2004
The type III effector protein AvrPto acts as a virulence factor in susceptible plants lacking a cognate resistance gene but triggers hypersensitive response and disease resistance in tomato plants carrying the Pto gene or in tobacco plants carrying an ...
Venkatappa K. Thara, Alexander R. Seilaniantz, Youping Deng, Yinghua Dong, Yinong Yang, Xiaoyan Tang, Jian-Min Zhou   +6 more
doaj   +1 more source

Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events

Scientific Reports, 2022
The full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants.
Hiroki Ura, Sumihito Togi, Yo Niida
doaj   +1 more source

A Nonpolymorphic Class I Gene in the Murine Major Histocompatibility Complex [PDF]

, 1984
DNA sequence analysis of a class I gene (QlO), which maps to the Qa2,3 locus in the C57BL/lO (H- 2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library.
Flavell, Richard A., Jay, Gilbert, Kress, Michael, Mellor, Andrew L., Weiss, Elisabeth H.   +4 more
core   +1 more source

Development of a Yeast Trihybrid Screen Using Stable Yeast Strains and Regulated Protein Expression

BioTechniques, 1998
We describe a yeast trihybrid system that facilitates rapid screening of cDNA libraries. Novel yeast vectors were developed that direct integration of cDNA encoding the bait and third protein component into the yeast chromosome.
K.J. Fuller, M.A. Morse, J.H.M. White, S.J. Dowell, M.J. Sims   +4 more
doaj   +1 more source

Display of a Maize cDNA library on baculovirus infected insect cells

BMC Biotechnology, 2008
Background Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies for efficient proteomic analysis ...
Jones Ian M, Chen Hongying, Fontaine Veronique, Meller Harel Helene, Millner Paul A   +4 more
doaj   +1 more source