Results 31 to 40 of about 420,567 (309)

cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]

, 2001
Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood, Irene M. Monahan, Joseph A. Mangan, Martin J. Everett, Ming-shi Li, Philip D. Butcher, Simon J. Waddell, Steve L. Martin   +7 more
core   +3 more sources

Genetic identity and differential gene expression between and [PDF]

, 2009
Background Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T.
Ashwini S Kucknoor, Vasanthakrishna Mundodi, JF Alderete   +2 more
core   +2 more sources

A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity

Neurobiology of Disease, 2001
Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial ...
Tanya Barrett, Tao Xie, Yulan Piao, Ora Dillon-Carter, George J. Kargul, Meng K. Lim, Francis J. Chrest, Robert Wersto, Daniel L. Rowley, Magdalena Juhaszova, Li Zhou, Marquis P. Vawter, Kevin G. Becker, Christopher Cheadle, William H. Wood, III, Una D. McCann, William J. Freed, Minoru S. Ko, George A. Ricaurte, David M. Donovan   +19 more
doaj   +1 more source

Assessment of Ion S5 NGS protocol for SARS-CoV-2 genome sequencing

Genetics & Applications, 2021
Monitoring of the lineages SARS-CoV-2 is equally important in a fight against COVID-19 epidemics, as is regular RT - PCR testing. Ion AmpliSeq Library kit plus is a robust and validated protocol for library preparation, but certain optimizations for
Dino Pećar, Ivana Čeko, Lana Salihefendić, Rijad Konjhodžić   +3 more
doaj   +1 more source

HC4 The Potential Impact Of Recommendations Made Through The Common Drug Review Program At The Canadian Agency For Drugs And Technologies In Health [PDF]

, 1990
A full-length cDNA clone specifying the nuclear-encoded subunit VIb of human cytochrome c oxidase (COX) was isolated from a human skeletal muscle cDNA expression library. This was done with antiserum directed against the group of subunits VIa, b and c of
AGSTERIBBE, E, BOLHUIS, PA, DAS, AT, DEVRIES, H, PONNE, NJ, SCHRAGE, C, TAANMAN, JW   +6 more
core   +2 more sources

Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]

, 2018
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane, Callewaert, Nico, De Koker, Andries   +2 more
core   +2 more sources

Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae

European Journal of Entomology, 2016
Macrocentrus cingulum is an important polyembryonic endoparasitic wasp that attacks larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) and the European corn borer, O. nubilalis (Hübner).
Tofael AHMED, Tian-Tao ZHANG, Zhen-Ying WANG, Kang-Lai HE, Shu-Xiong BAI   +4 more
doaj   +1 more source

Cloning of terminal transferase cDNA by antibody screening [PDF]

, 1984
A cDNA library was prepared from a terminal deoxynucleotidyltransferase-containing thymoma in the phage vector λgt11. By screening plaques with anti-terminal transferase antibody, positive clones were identified of which some had β-galactosidase-cDNA ...
Baltimore, David, Landau, Nathaniel R., Silverstone, Allen E., St. John, Thomas P., Weissman, Irving L., Wolf, Susan C.   +5 more
core  

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

Colorectal cancer‐derived FGF19 is a metabolically active serum biomarker that exerts enteroendocrine effects on mouse liver

Molecular Oncology, EarlyView.
Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley, Michael W. Rohr, Joseph A. Goode, Sai Preethi Nakkina, Jignesh G. Parikh, Deborah A. Altomare   +5 more
wiley   +1 more source