Results 31 to 40 of about 804,751 (297)

Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali. [PDF]

PLoS ONE, 2011
Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis.
Takeshi Terahara, Seinen Chow, Hiroaki Kurogi, Sun-Hee Lee, Katsumi Tsukamoto, Noritaka Mochioka, Hideki Tanaka, Haruko Takeyama   +7 more
doaj   +1 more source

Method of identifying inhibitors of glutathione S-transferase (GST) gene expression [PDF]

, 1999
Complementary DNA and genomic clones for three variants of GST-.pi. are disclosed. It is demonstrated that certain of these variants are overexpressed in gliomas, thereby indicating an involvement with that form of cancer.
Akande, Olanike, Ali-Osman, Francis, Antoun, Gamil, Buolamwini, John K., Keller, Charles W., Lo, Hui-Wen, Lopez-Berestein, Gabriel   +6 more
core   +1 more source

Sequence sensitivity of breathing dynamics in heteropolymer DNA

, 2006
We study the fluctuation dynamics of localized denaturation bubbles in heteropolymer DNA with a master equation and complementary stochastic simulation based on novel DNA stability data.
B. Alberts, D. Poland, N. G. van Kampen, Oleg Krichevsky, Ralf Metzler, S. G. Delcourt, Suman K. Banik, Tobias Ambjörnsson   +7 more
core   +1 more source

RNA-programmed genome editing in human cells

eLife, 2013
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek, Alexandra East, Aaron Cheng, Steven Lin, Enbo Ma, Jennifer Doudna   +5 more
doaj   +1 more source

Lipid bilayer-assisted dynamic self-assembly of hexagonal DNA origami blocks into monolayer crystalline structures with designed geometries

iScience, 2022
Summary: The DNA origami technique is used to construct custom-shaped nanostructures that can be used as components of two-dimensional crystalline structures with user-defined structural patterns.
Yuki Suzuki, Ibuki Kawamata, Kotaro Watanabe, Eriko Mano   +3 more
doaj   +1 more source

Simplified detection of the hybridized DNA using a graphene field effect transistor

Science and Technology of Advanced Materials, 2017
Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA).
Arun Kumar Manoharan, Shanmugavel Chinnathambi, Ramasamy Jayavel, Nobutaka Hanagata   +3 more
doaj   +1 more source

Molecular Spiders with Memory [PDF]

, 2007
Synthetic bio-molecular spiders with "legs" made of single-stranded segments of DNA can move on a surface which is also covered by single-stranded segments of DNA complementary to the leg DNA.
G. H. Weiss, I. Benjamini, J. Howard, M. P. W. Zerner, N. G. Van Kampen, P. L. Krapivsky, S. Klumpp, S. Redner, Tibor Antal   +8 more
core   +1 more source

Ion-Channel Genosensor for the Detection of Specific DNA Sequences Derived from Plum Pox Virus in Plant Extracts

Sensors, 2014
A DNA biosensor for detection of specific oligonucleotides sequences of Plum Pox Virus (PPV) in plant extracts and buffer is proposed. The working principles of a genosensor are based on the ion-channel mechanism. The NH2-ssDNA probe was deposited onto a
Kamila Malecka, Lech Michalczuk, Hanna Radecka, Jerzy Radecki   +3 more
doaj   +1 more source

The process of infection with bacteriophage phiX174, XXX. Replication of double-stranded phiX DNA [PDF]

, 1969
Intermediates involved in the replication of double-stranded phiX174 RF DNA have been identified and partially characterized. Analysis of pulse-labeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of ...
Knippers, Rolf, Sinsheimer, Robert L., Whalley, J. Millar   +2 more
core  

Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus

Sensors, 2013
A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution ...
Chee-Seng Toh, Jiajia Deng
doaj   +1 more source