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Gene Expression Analysis in the Pneumococcus
2019Bacterial cells modify their gene expression profiles throughout different stages of growth and in response to environmental cues. Analyses of gene expression across conditions reveal both conserved and condition-specific gene responses of bacteria to adapt to these dynamic conditions.
Rory A, Eutsey +4 more
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Introduction to the Gene Expression Analysis
2016In 1941, Beadle and Tatum published experiments that would explain the basis of the central dogma of molecular biology, whereby the DNA through an intermediate molecule, called RNA, results proteins that perform the functions in cells. Currently, biomedical research attempts to explain the mechanisms by which develops a particular disease, for this ...
San Segundo Val, Ignacio +1 more
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Serial Analysis of Gene Expression
Science, 1995The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and
V E, Velculescu +3 more
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Analysis of Gene Expression by PCR
2003Methods used to study gene expression rely on two basic conditions. First, sufficient tissue must be available for analysis, and second, the gene must be expressed at a level high enough to be detectable by the method used. Where the gene of interest is expressed in small tissues or groups of cells, for example, in mouse embryos, or where transcripts ...
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Analysis of Mitochondrial Gene Expression
2013Dictyostelium provides a well-established model system for the study of mitochondrial biology and disease. A complete mitochondrial transcription and RNA-processing map has been generated, while the start site for transcription and the responsible RNA polymerase have also been identified, as have the major cotranscriptional cleavage sites that generate
Jessica E, Accari +3 more
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Analysis of Cytokine Expression in Dermatology
Archives of Dermatology, 2002During the past decade, the detection of cytokines has been a focus of scientific interest, including in dermatology. Dysregulation of cytokine production seems to be involved in the pathogenesis of various diseases. The determination of cytokine levels is of increasing diagnostic importance, and cytokines are used as therapeutic agents too.
Khusru, Asadullah +2 more
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Factor Analysis of Expressions of Anxiety
Psychological Reports, 197840 female college students were videotaped in an interview during which they were questioned about sexual attitudes. Independent pairs of judges rated subjects using these videotapes. A rotated factor analysis performed on 18 non-verbal ratings, a finger-sweat index, the subject's subjective rating of own anxiety, and the Taylor Manifest Anxiety Scale ...
A P, Jurich, J A, Jurich
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Northern Analysis of Gene Expression
2013Northern analysis detects and quantitates full-length RNA transcripts by hybridizing a labeled sequence-specific probe to RNA fractionated by size and blotted onto a membrane. The first step in Northern analysis is RNA extraction from the tissue(s) of interest, followed by RNA denaturation and electrophoresis to separate all RNA transcripts in the ...
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Sonological analysis of clarinet expressivity
1980Seven different performances of a fragment of W. A. Mozart's Concert for Clarinet (K622) were recorded. Each performance was inspired giving the player a sensorial type adjective in order to suggest various kinds of expressive intentions. Certain particularly significant sonological parameters were then measured.
CANAZZA TARGON, SERGIO +3 more
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Serial analysis of gene expression
Nature Protocols, 2006Serial analysis of gene expression (SAGE) is a method used to obtain comprehensive, unbiased and quantitative gene-expression profiles. Its major advantage over arrays is that it does not require a priori knowledge of the genes to be analyzed and reflects absolute mRNA levels. Since the original SAGE protocol was developed in a short-tag (10-bp) format,
Min, Hu, Kornelia, Polyak
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