Results 91 to 100 of about 1,656,664 (330)

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Hierarchical Multi-Bottleneck Classification Method And Its Application to DNA Microarray Expression Data [PDF]

, 2003
The recent development of DNA microarray technology is creating a wealth of gene expression data. Typically these datasets have high dimensionality and a lot of varieties.
Hsu, Wen Jing, Wong, Weng Fai, Xiong, Xuejian   +2 more
core  

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Codes et intelligences : externalisation, retour sur soi et brouillage des frontières entre nature et culture

Sociologies, 2019
The article retains four types of codes and intelligences, according to their historical order of appearance: 1) DNA codes, associated with genomic intelligence, 2) neural codes, associated with sensory-motor intelligence, 3) linguistic codes, associated
André Petitat
doaj  

A Novice-Friendly and Accessible Networked Educational Robotics Simulation Platform

Education Sciences
Despite its potential for STEM education, educational robotics remains out of reach for many classrooms due to upfront purchase costs, maintenance requirements, storage space, and numerous other barriers to entry.
Gordon Stein, Devin Jean, Saman Kittani, Menton Deweese, Ákos Lédeczi   +4 more
doaj   +1 more source

Non-polynomial Worst-Case Analysis of Recursive Programs

, 2017
We study the problem of developing efficient approaches for proving worst-case bounds of non-deterministic recursive programs. Ranking functions are sound and complete for proving termination and worst-case bounds of nonrecursive programs.
A Chakarov, A Podelski, A Schrijver, A Schrijver, AR Bradley, B Cook, B Cook, BS Gulavani, C Alias, C Urban, CS Lee, D Handelman, E Albert, E Albert, E Albert, J Farkas, J Hoffmann, J Hoffmann, J Hoffmann, J Hoffmann, K Chatterjee, L Shen, L Yang, M Brockschmidt, M Hofmann, M Hofmann, M Sinn, MA Colón, MA Colón, O Bournez, O Shkaravska, P Cousot, P Flajolet, R Alur, R Wilhelm, RG Bartle, RW Floyd, S Gulwani, S Jost, T Kuwahara, TH Cormen, W Chin, WH Hesselink   +42 more
core   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Expressing aspectual interactions in requirements engineering: Experiences, problems and solutions

Science of Computer Programming, 2012
AbstractAspect Oriented Requirements Engineering (AORE) provides support for modularizing crosscutting requirements. In the context of an industrial project in the domain of Slot Machines we needed to perform AORE, with a special emphasis on dependencies and interactions among concerns.
Zambrano Polo y La Borda, Arturo Federico, Fabry, Johan, Gordillo, Silvia Ethel   +2 more
openaire   +5 more sources

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

Cyclic nucleotide signaling as a drug target in retinitis pigmentosa

FEBS Letters, EarlyView.
Disruptions in cGMP and cAMP signaling can contribute to retinal dysfunction and photoreceptor loss in retinitis pigmentosa. This perspective examines the mechanisms and evaluates emerging evidence on targeting these pathways as a potential therapeutic strategy to slow or prevent retinal degeneration.
Katri Vainionpää, Ahmed B. Montaser, Henri Leinonen   +2 more
wiley   +1 more source