Results 21 to 30 of about 327,732 (266)

Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Microbial Cell Factories, 2021
Background Codon optimization is a common method to improve protein expression levels in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to match the codon usage bias.
Yide Huang, Ting Lin, Lingfang Lu, Fan Cai, Jie Lin, Yu′e Jiang, Yao Lin   +6 more
doaj   +1 more source

Optimization of expression and purification of recombinant S1 domain of the porcine epidemic diarrhea virus spike (PEDV- S1) protein in Escherichia coli

Biotechnology & Biotechnological Equipment, 2017
The PEDV-S1 protein, one of the members of a large family of PEDV, is significantly upregulated and has been targeted as a biomarker of cellular stress in several studies.
Nguyen Van Noi, Yao-Chi Chung
doaj   +1 more source

Accuracy and Inter-Unit Reliability of Ultra-Wide-Band Tracking System in Indoor Exercise

Applied Sciences, 2019
The purpose of this study was to assess the accuracy of positional data and the inter-unit reliability of an ultra-wide-band (UWB) tracking system. Four well-trained males performed five courses designed for the analysis of x- and y-coordinate accuracy ...
Alejandro Bastida-Castillo, Carlos David Gómez-Carmona, Ernesto De la Cruz-Sánchez, Xavier Reche-Royo, Sergio José Ibáñez, José Pino Ortega   +5 more
doaj   +1 more source

Examining the Effects of Altitude on Workload Demands in Professional Basketball Players during the Preseason Phase

Sensors
Basketball involves frequent high-intensity movements requiring optimal aerobic power. Altitude training can enhance physiological adaptations, but research examining its effects in basketball is limited.
Sergio J. Ibáñez, Carlos D. Gómez-Carmona, Sergio González-Espinosa, David Mancha-Triguero   +3 more
doaj   +1 more source

Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

Frontiers in Microbiology, 2019
Laccase is one of the oldest known and intensively studied fungal enzymes capable of oxidizing recalcitrant lignin-resembling phenolic compounds. It is currently well established that fungal genomes almost always contain several non-allelic copies of ...
Olga S. Savinova, Konstantin V. Moiseenko, Ekaterina A. Vavilova, Andrey M. Chulkin, Tatiana V. Fedorova, Tatiana V. Tyazhelova, Daria V. Vasina   +6 more
doaj   +1 more source

A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor [PDF]

Open Biology, 2012
Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei.
S. K. Poon, L. Peacock, W. Gibson, K. Gull, S. Kelly   +4 more
doaj   +1 more source

Intensity Thresholds for External Workload Demands in Basketball: Is Individualization Based on Playing Positions Necessary?

Sensors
Currently, basketball teams use inertial devices for monitoring external and internal workload demands during training and competitions. However, the intensity thresholds preset by device manufacturers are generic and not adapted for specific sports (e.g.
Sergio J. Ibáñez, Carlos D. Gómez-Carmona, Pablo López-Sierra, Sebastián Feu   +3 more
doaj   +1 more source

Anthropometric Influence on Preschool Children’s Physical Fitness and Motor Skills: A Systematic Review

Journal of Functional Morphology and Kinesiology
Early childhood is a critical period for physical and motor development with implications for long-term health. This systematic review examined the relationship between anthropometric characteristics and measures of physical fitness and motor skills in ...
Markel Rico-González, Luca Paolo Ardigò, Ana P. Ramírez-Arroyo, Carlos D. Gómez-Carmona   +3 more
doaj   +1 more source

Referral Patterns and Diagnostic Timeliness in Pediatric Cancer: A Hospital‐Based Study in Indonesia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Timely diagnosis and treatment are critical for improving survival among children with cancer. In low‐ and middle‐income countries (LMICs), delays are common and may be influenced by fragmented referral pathways and diagnostic limitations.
Nur Melani Sari, Regina Hadi Putri, Fatimah Assyifa, Judith Spijkerman, Gertjan Johannes Kaspers, Susi Susanah   +5 more
wiley   +1 more source

Physical Workload Patterns in U-18 Basketball Using LPS and MEMS Data: A Principal Component Analysis by Quarter and Playing Position

Sensors
Basketball is a high-intensity, intermittent sport in which physical demands fluctuate depending on different contextual variables. Most studies addressed these demands in isolation without integrative approaches.
Sergio J. Ibáñez, Markel Rico-González, Carlos D. Gómez-Carmona, José Pino-Ortega   +3 more
doaj   +1 more source