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Extending the depth of field in microscopy: A review
2016 International Conference on Image and Vision Computing New Zealand (IVCNZ), 2016This paper investigates multi-focus image fusion methods in the context of microscope. Limited depth of field is one of the major limitations in microscopy. Objects which are thicker than the depth of field cannot be captured completely within one sharp image.
Anoop Suraj Ambikumar +2 more
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X-ray ptychography with extended depth of field
Optics Express, 2016 Ptychographic X-ray computed tomography is a coherent diffractive imaging method that offers nanometer-scale resolution with quantitative contrast. It offers the possibility to study relatively thick samples by using high energy X-ray photons and exploiting the phase contrast.
Andreas Menzel +2 more
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Asymmetric phase masks for extended depth of field
Applied Optics, 2004We present a family of asymmetric phase masks that extends the depth of field of an optical system. To verify our proposal, we compute several modulation transfer functions with focus errors, and we report numerical simulations of the images that can be achieved by use of our proposed procedure.
Jorge Ojeda-Castañeda, Albertina Castro
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Virtual Microscopy with Extended Depth of Field
Digital Image Computing: Techniques and Applications (DICTA'05), 2005In this paper, we describe a virtual microscope system, based on JPEG 2000, which utilizes extended depth of field (EDF) imaging. Through a series of observer trials we show that EDF imaging improves both the local image quality of individual fields of view (FOV) and the accuracy with which the FOVs can be mosaiced (stitched) together.
Andrew P. Bradley +2 more
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Extended depth of field by colored apodization
Optics Letters, 2011We describe an approach to achieving extended depth of field for a camera system based on reducing the aperture of the blue channel of a three-color camera by apodizing the lens with a yellow-colored filter. The resulting improvement in depth of field for the blue channel allows red and green channels to be digitally postprocessed for improved ...
Andrew, Kay +2 more
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Extended depth-of-field microscopy
SPIE Proceedings, 2010In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a nearisotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations.
Christopher Smith +4 more
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Extended Depth of Field in Microscope
Optics in the Life Sciences, 2013A cubic phase mask is used in an infinity microscope objective to extend depth of field. The mask is placed between the objective and tube lens, so that neither objective nor tube lens needs redesigning.
Tingyu Zhao, Guoqiang Li
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Extending depth of field in noisy light field photography
2017 IEEE 8th International Conference on Awareness Science and Technology (iCAST), 2017A robust depth of field (DOF) extension algorithm was proposed based on the refocusing property of a light field photograph and the depth-from-defocus approach of multi-focus image fusing. The main techniques of the algorithm are depth estimation and all-in-focus image estimation. By making use of the redundancy of a light field photograph, we leverage
Shih-Shuo Tung +2 more
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Extended depth of field through an axicon
Journal of Modern Optics, 2009One of the main disadvantages of imaging systems is the limited depth of field. We present the ability of a refractive axicon for imaging with an extended depth of field. We design an imaging system with a two-step imaging approach that uses a CCD camera to capture intermediate images and uses a digital process to obtain the final images.
QINGHUA Lyu, Xuanze Wang, Yuning Zhong
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Multiple-frame photography for extended depth of field
Applied Optics, 2013For extending the depth of field, we analyze the result of superimposing several snapshots, which are taken while changing the amount of focus error, at full pupil aperture. We unveil the use of a varifocal lens for controlling the amount of focus error, without modifying either the lateral magnification or light throughput.
Jorge, Ojeda-Castañeda +2 more
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