Results 111 to 120 of about 429,469 (300)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Catar: un año de bloqueo

open access: yes, 2018
El cuarteto árabe no ha logrado modificar el liderazgo regional ni la política exterior de Catar, que se ha adaptado a la nueva situación tejiendo complicidades con actores claves en Oriente Próximo, principalmente Irán y ...
Álvarez-Ossorio Alvariño, Ignacio
core   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

open access: yesFEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell   +8 more
wiley   +1 more source

A análise do comércio exterior brasileiro de 1995 até 2010 [PDF]

open access: yes, 2016
TCC (graduação) - Universidade Federal de Santa Catarina. Centro Sócio-Econômico. Economia.A abertura econômica promovida pelo Brasil a partir da década 1990 provocou mudanças no mercado interno, no desenvolvimento do país e também no comércio exterior ...
Fuganti, Paulo Victor Caetano
core  

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

open access: yesFEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss   +7 more
wiley   +1 more source

Especialización en banca (Comercio exterior) [PDF]

open access: yes, 1980
Tercera unidad de la serie Especialización en banca en el que se describen generalidades asociadas al comercio exterior, se recuperan aspectos históricos, se define el concepto de balanza de pagos, importaciones, exportaciones, arancel y comercio ...

core  

Envelopes and exteriority

open access: yesFormAkademisk - forskningstidsskrift for design og designdidaktikk, 2019
This article discusses the relationship of architecture to its surrounding environment. The objective of the article is i) to reposition the relationship of architecture and its surrounding exterior by expanding the understanding of architectural envelopes, and ii) to systematically define exterior space as design input.
openaire   +2 more sources

Effect of terahertz irradiation on DNA damage repair in living cells

open access: yesFEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno   +3 more
wiley   +1 more source

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