Results 61 to 70 of about 642,758 (265)

NGS-Based S. aureus Typing and Outbreak Analysis in Clinical Microbiology Laboratories: Lessons Learned From a Swiss-Wide Proficiency Test

Frontiers in Microbiology, 2020
Whole genome sequencing (WGS) enables high resolution typing of bacteria up to the single nucleotide polymorphism (SNP) level. WGS is used in clinical microbiology laboratories for infection control, molecular surveillance and outbreak analyses.
David Dylus, David Dylus, David Dylus, Trestan Pillonel, Onya Opota, Daniel Wüthrich, Daniel Wüthrich, Helena M. B. Seth-Smith, Helena M. B. Seth-Smith, Adrian Egli, Adrian Egli, Stefano Leo, Vladimir Lazarevic, Jacques Schrenzel, Sacha Laurent, Claire Bertelli, Dominique S. Blanc, Stefan Neuenschwander, Alban Ramette, Laurent Falquet, Laurent Falquet, Frank Imkamp, Peter M. Keller, Andre Kahles, Andre Kahles, Simone Oberhaensli, Simone Oberhaensli, Valérie Barbié, Christophe Dessimoz, Christophe Dessimoz, Christophe Dessimoz, Christophe Dessimoz, Christophe Dessimoz, Gilbert Greub, Aitana Lebrand   +34 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Excellent capability for molecular detection of Aedes-borne dengue, Zika, and chikungunya viruses but with a need for increased capacity for yellow fever and Japanese encephalitis viruses: an external quality assessment in 36 European laboratories

Journal of Clinical Microbiology
Mosquito-borne viruses represent a large global health burden. With geographic expansion of competent vectors for chikungunya virus (CHIKV), dengue virus (DENV), and Zika virus (ZIKV) in Europe, it is anticipated that the number of autochthonous cases of
Lance D. Presser, Cécile Baronti, Ramona Moegling, Laura Pezzi, Yaniv Lustig, Céline M. Gossner, Chantal B. E. M. Reusken, Rémi N. Charrel   +7 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Performance of a quality control center supporting national antimicrobial resistance surveillance

British Journal of Biomedical Science
IntroductionEnsuring the reliability, standardization, and international comparability of antimicrobial resistance (AMR) surveillance data critically depends on the implementation of robust quality assurance frameworks.
Dong Woo Shin, Hyunji Kim, Jeong Su Park, Kyoung Un Park, Kyoung Un Park, Min Hyuk Choi, Dokyun Kim, Seok Hoon Jeong, Hee Jung Kim, Young Ah Kim, Kwangjin Ahn, Young Uh, Yong Jun Kwon, Jong Hee Shin, Soo Hyun Kim, Jeong Hwan Shin, Hee Young Kang, Dong Chan Moon, Sung Young Lee, Songmee Bae   +19 more
doaj   +1 more source

Dry Panels Supporting External Quality Assessment Programs for Next Generation Sequencing-Based HIV Drug Resistance Testing

Viruses, 2020
External quality assessment (EQA) is a keystone element in the validation and implementation of next generation sequencing (NGS)-based HIV drug resistance testing (DRT). Software validation and evaluation is a critical element in NGS EQA programs.
Marc Noguera-Julian, Emma R. Lee, Robert W. Shafer, Rami Kantor, Hezhao Ji   +4 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Quality assessment: importance of external evaluation and monitoring

Avances en Supervisión Educativa, 2007
The aim of this paper is to illustrate the steps to develop a quality assessment and analyse the importance of external assessment and follow-up in a university.
Juan José Tarí Guilló, Susana Amalia de Juana Espinosa, Jerónimo Manuel Mora Pascual   +2 more
doaj  

Evaluation of the results of MOTAKK hepatitis C virus RNA genotyping and hepatitis delta virus external quality assessment programs during 2015-2016

The Turkish Journal of Gastroenterology, 2019
Background/Aims: To evaluate the HCV RNA genotyping and HDV RNA tests that are performed in molecular microbiology laboratories in Turkey as part of a national external quality assessment programme, MOTAKK (Moleküler Tanıda Kalite Kontrol) (English ...
Ersin Karataylı, Ege Soydemir, Zeynep Büşra Aksoy, Mehtap Kızılpınar, Aylin Altay Koçak, Senem Ceren Karataylı, Esra Yurdcu, Umut Yıldırım, Haluk Güriz, Gülendam Bozdayı, Gülendam BOZDAYI, Osman İlhan, Yasin Yıldırım, MOTAKK HCV Genotype and HDV Study Group, A. Mithat Bozdayı   +14 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source