Results 41 to 50 of about 935,910 (334)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

The Extinction and De-Extinction of Species

open access: yesPhilosophy & Technology, 2016
In this paper, we discuss the following four alternative ways of understanding the outcomes of resurrection biology (also known as de-extinction). Implications of each of the ways are discussed with respect to concepts of species and extinction. (1) Replication: animals created by resurrection biology do not belong to the original species but are ...
Siipi, Helena, Finkelman, Leonard
openaire   +2 more sources

Partial extinction and reinstatement [PDF]

open access: yes, 2018
Fear extinction is not permanent but more vulnerable than the original fear memory, as relapse phenomena have traditionally shown. Partial extinction has been proposed as a strategy that may serve to mitigate relapses. Partial extinction differs from the
Flores, Amanda   +4 more
core  

The mid-infrared extinction law in the darkest cores of the Pipe Nebula

open access: yes, 2012
Context. The properties of dust grains, in particular their size distribution, are expected to differ from the interstellar medium to the high-density regions within molecular clouds. Aims.
Alves, J.   +4 more
core   +1 more source

The Extinction Towards the GRB970228 Field [PDF]

open access: yes, 1998
We determine the local galactic extinction towards the field of gamma-ray burst GRB970228 using a variety of methods. We develop a maximum likelihood method for measuring the extinction by comparing galaxy counts in the field of interest to those in a ...
Brainerd T. G.   +8 more
core   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

The conservation impact of botanical drones: Documenting and collecting rare plants from vertical cliffs and other hard‐to‐reach areas

open access: yesEcological Solutions and Evidence
A high percentage of island floras are at risk of extinction and have been reduced to relic populations, often in remote hard‐to‐reach areas. Uncrewed aircraft systems (UAS aka drones) are now being utilized to assist in the survey and collection of rare
Ben Nyberg   +12 more
doaj   +1 more source

Estradiol reduction through aromatase inhibition impairs cocaine seeking in male rats

open access: yesFrontiers in Behavioral Neuroscience
IntroductionClinical and preclinical research on cocaine use disorder (CUD) has shown that sex differences in drug seeking are influenced by hormonal fluctuations.
John K. Alvarado-Torres   +7 more
doaj   +1 more source

The Optical -- Mid-infrared Extinction Law of the l=165 Sightline in the Galactic Plane: Diversity of Extinction Law in the Diffuse Interstellar Medium

open access: yes, 2017
Understanding the effects of dust extinction is important to properly interpret observations. The optical total-to-selective extinction ratio, Rv = Av/E(B-V), is widely used to describe extinction variations in ultraviolet and optical bands. Since the Rv=
Chen, Xiaodian   +4 more
core   +2 more sources

Mid-Infrared Extinction and its Variation with Galactic Longitude [PDF]

open access: yes, 2009
Based on the data obtained from the Spitzer/GLIPMSE Legacy Program and the 2MASS project, we derive the extinction in the four IRAC bands, [3.6], [4.5], [5.8] and [8.0] micron, relative to the 2MASS Ks band (at 2.16 micron) for 131 GLIPMSE fields along ...
Aigen Li   +42 more
core   +2 more sources

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