3D‐Printed Porous Hydroxyapatite Formed via Enzymatic Mineralization
Bone combines lightness, strength, and the ability to heal, inspiring new materials design. This work introduces a room‐temperature, enzyme‐mediated 3D printing method to create porous hydroxyapatite scaffolds. The process avoids energy‐intensive sintering, preserves bioactivity, and allows control over porosity and mineralization.
Francesca Bono +6 more
wiley +1 more source
Streamline Protocol for <i>Arabidopsis</i> Apoplastic Fluid Isolation Enables a Detailed Proteomic View of the Plant Extracellular Space. [PDF]
Chen KE, Karinshak M, Vierstra RD.
europepmc +1 more source
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian +5 more
wiley +1 more source
Disruption of Extracellular Matrix and Perineuronal Nets Modulates Extracellular Space Volume and Geometry. [PDF]
Syková E +13 more
europepmc +1 more source
Unveiling Multidimensional Physicochemical Design Principles for Tissue Processing Hydrogels
This study establishes a materials‐based design framework for polymer hydrogels in tissue clearing, linking physicochemical properties to performance in tissue processing, labeling, and imaging. By analyzing rheology, swelling, porosity, antibody diffusion, mechanical performance, and thermochemical stability across platforms, this work provides a ...
Sangjae Kim +8 more
wiley +1 more source
Glucose uptake is essential for <i>Brucella abortus</i> growth in the extracellular space of the murine placenta. [PDF]
Lázaro-Antón L +7 more
europepmc +1 more source
Massive efflux of adenosine triphosphate into the extracellular space immediately after experimental traumatic brain injury. [PDF]
Moro N, Ghavim SS, Sutton RL.
europepmc +1 more source
Bioinspired Stabilization of Fluorescent Au@SiO2 Tracers for Multimodal Biological Imaging
This study demonstrates a bioinspired stabilization strategy for fluorescent gold‐silica nanoparticles. Inspired by natural biosilica maturation, high‐temperature calcination transforms the silica shells, preventing dissolution in cell culture media and intracellular environments.
Wang Sik Lee +5 more
wiley +1 more source
Real-time imaging of glutamate transients in the extracellular space of acute human brain slices using a single-wavelength glutamate fluorescence nanosensor. [PDF]
Brandner S +7 more
europepmc +1 more source
Quantum sensing reveals intricate patterns linking endo‐lysosomal maturation to cardiac fibrosis progression, highlighting complexity in cellular remodeling. This study investigates fibroblast‐to‐myofibroblast transition under cell aging, stiffness, and TGF‐β stimulation, comparing nanodiamond uptake, endo‐lysosomal dynamics, and free radical ...
Aldona Mzyk +3 more
wiley +1 more source

