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Purification of eucaryotic extrachromosomal circular DNAs using exonuclease III
Analytical Biochemistry, 1990A method for the isolation of eucaryotic extrachromosomal circular (ecc) DNA is described. Exonuclease III was used to preparatively digest linear and open circular forms of DNA; the resultant exonuclease-resistant molecules were then characterized by buoyant density gradient sedimentation and were found to be essentially covalently closed circular DNA.
James W. Gaubatz, Sonia C. Flores
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Extrachromosomal circular DNAs from murine hemopoietic tissue cells
Plasmid, 1983Extrachromosomal circular DNA complexes from cells of murine hemopoietic organs, bone marrow, thymus, spleen, and lymph nodes were examined by mica-press-adsorption method (H. Yamagishi, T. Kunisada, and T. Tsuda, 1982, Plasmid 8, 299-306). They showed wide size distribution, from 0.3 to 10 micron.
Yasuyuki Yamada +5 more
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Extrachromosomal circular DNAs and genomic sequence plasticity in eukaryotic cells
Mutation Research/DNAging, 1990The ability of eukaryotic organisms of the same genotype to vary in developmental pattern or in phenotype according to varying environmental conditions is frequently associated with changes in extrachromosomal circular DNA (eccDNA) sequences. Although variable in size, sequence complexity, and copy number, the best characterized of these eccDNAs ...
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Intragenic amplification and formation of extrachromosomal small circular DNA
2002The PIP gene is expressed in exocrine glands and, in pathologic conditions, in breast cysts and breast cancers exhibiting apocrine features. It is localized on the long arm of chromosome 7, a region frequently alterated in mammary tumors. We previously described an abnormal restriction pattern of the PIP gene in 33% of prostate carcinomas analyzed ...
Autiero M +10 more
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