Results 41 to 50 of about 2,048,993 (218)

Studying extrachromosomal DNA with the ecTag method

Nature Reviews Cancer, 2022
Eunhee Yi
semanticscholar   +4 more sources

Microhomology-mediated circular DNA formation from oligonucleosomal fragments during spermatogenesis

eLife, 2023
The landscape of extrachromosomal circular DNA (eccDNA) during mammalian spermatogenesis, as well as the biogenesis mechanism, remains to be explored. Here, we revealed widespread eccDNA formation in human sperms and mouse spermatogenesis.
Jun Hu, Zhe Zhang, Sai Xiao, Yalei Cao, Yinghong Chen, Jiaming Weng, Hui Jiang, Wei Li, Jia-Yu Chen, Chao Liu   +9 more
doaj   +1 more source

Identification of Extrachromosomal Linear microDNAs Interacted with microRNAs in the Cell Nuclei

Cells, 2019
Extrachromosomal DNA exists in two forms: Covalently closed circular and linear. While diverse types of circular extrachromosomal DNA have been identified with validated in vivo functions, little is known about linear extrachromosomal DNA. In this study,
Teng Sun, Kun Wang, Cuiyun Liu, Yin Wang, Jianxun Wang, Peifeng Li   +5 more
doaj   +1 more source

Extrachromosomal DNA formation enables tumor immune escape potentially through regulating antigen presentation gene expression

Scientific Reports, 2022
Extrachromosomal DNA (ecDNA) is a type of circular and tumor specific genetic element. EcDNA has been reported to display open chromatin structure, facilitate oncogene amplification and genetic material unequal segregation, and is associated with poor ...
Tao Wu, Chenxu Wu, Xiangyu Zhao, Guangshuai Wang, W. Ning, Ziyu Tao, Fuxiang Chen, Xue-song Liu   +7 more
semanticscholar   +1 more source

Isolation of Extrachromosomal Elements by Histone Immunoprecipitation

BioTechniques, 2001
Here, we describe a gentle and effective method for the rapid and reproducible isolation of histone-bound extrachromosomal DNA molecules called extrachromosomal elements (EEs). This method facilitates the harvest of a specific population of EEs following
T.I. Kuschak, B.C. Kuschak, G.M. Smith, J.A. Wright, S. Mai   +4 more
doaj   +1 more source

Oncogene expression from extrachromosomal DNA is driven by copy number amplification and does not require spatial clustering in glioblastoma stem cells

bioRxiv, 2022
Extrachromosomal DNA (ecDNA) are frequently observed in human cancers and are responsible for high levels of oncogene expression. In glioblastoma (GBM), ecDNA copy number correlates with poor prognosis. It is hypothesized that their copy number, size and
K. Purshouse, E. Friman, S. Boyle, P. Dewari, Vivien Grant, A. Hamdan, G. Morrison, P. Brennan, S. Beentjes, S. Pollard, W. Bickmore   +10 more
semanticscholar   +1 more source

Extrachromosomal DNA: An Emerging Hallmark in Human Cancer.

Annual Review of Pathology, 2021
Human genes are arranged on 23 pairs of chromosomes, but in cancer, tumor-promoting genes and regulatory elements can free themselves from chromosomes and relocate to circular, extrachromosomal pieces of DNA (ecDNA).
Sihan Wu, V. Bafna, Howard Y. Chang, P. Mischel   +3 more
semanticscholar   +1 more source

Pioneering insights of extrachromosomal DNA (ecDNA) generation, action and its implications for cancer therapy

International Journal on Biological Sciences, 2022
Extrachromosomal DNA (ecDNA) is a cancer-specific circular DNA molecule that is derived from chromosomes. In contrast with linear chromosomes, ecDNA exhibits a unique structure that can be representative of high chromosome accessibility, contributing to ...
Zesheng Li, Bo Wang, Hao Liang, Lei Han
semanticscholar   +1 more source

Formation of Extrachromosomal Circular DNA from Long Terminal Repeats of Retrotransposons in Saccharomyces cerevisiae

G3: Genes, Genomes, Genetics, 2016
Extrachromosomal circular DNA (eccDNA) derived from chromosomal Ty retrotransposons in yeast can be generated in multiple ways. Ty eccDNA can arise from the circularization of extrachromosomal linear DNA during the transpositional life cycle of ...
Henrik D. Møller, Camilla E. Larsen, Lance Parsons, Anders Johannes Hansen, Birgitte Regenberg, Tobias Mourier   +5 more
doaj   +1 more source

In vivo functional genomic studies of sterol carrier protein-2 gene in the yellow fever mosquito. [PDF]

PLoS ONE, 2011
A simple and efficient DNA delivery method to introduce extrachromosomal DNA into mosquito embryos would significantly aid functional genomic studies. The conventional method for delivery of DNA into insects is to inject the DNA directly into the embryos.
Rong Peng, Vilena I Maklokova, Jayadevi H Chandrashekhar, Que Lan   +3 more
doaj   +1 more source