Results 61 to 70 of about 2,048,993 (218)

Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Molecular Therapy: Methods & Clinical Development, 2016
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy, Chintan D Vin, Megan M Slough, Wayne R Gombotz, Brenna Kelley-Clarke   +4 more
doaj   +1 more source

Gene Amplification and the Extrachromosomal Circular DNA [PDF]

Genes, 2021
Oncogene amplification is closely linked to the pathogenesis of a broad spectrum of human malignant tumors. The amplified genes localize either to the extrachromosomal circular DNA, which has been referred to as cytogenetically visible double minutes (DMs), or submicroscopic episome, or to the chromosomal homogeneously staining region (HSR).
openaire   +2 more sources

Telomere damage induces internal loops that generate telomeric circles

Nature Communications, 2020
Extrachromosomal circular DNA made of telomeric repeats have been found to have an effect on telomere maintenance. By combining electron microscopy with a telomere purification procedure the authors identify damage-induced i-loops as a key intermediate ...
Giulia Mazzucco, Armela Huda, Martina Galli, Daniele Piccini, Michele Giannattasio, Fabio Pessina, Ylli Doksani   +6 more
doaj   +1 more source

Extrachromosomal DNA amplicons in antimalarial‐resistant Plasmodium falciparum [PDF]

Molecular Microbiology, 2020
AbstractExtrachromosomal (ec) DNAs are genetic elements that exist separately from the genome. Since ecDNA can carry beneficial genes, they are a powerful adaptive mechanism in cancers and many pathogens. For the first time, we report ecDNA contributing to antimalarial resistance in Plasmodium falciparum, the most virulent human malaria parasite. Using
Jennifer M. McDaniels, Adam C. Huckaby, Sabrina A. Carter, Sabrina Lingeman, Audrey Francis, Molly Congdon, Webster Santos, Pradipsinh K. Rathod, Jennifer L. Guler   +8 more
openaire   +2 more sources

Disentangling oncogenic amplicons in esophageal adenocarcinoma

Nature Communications
Esophageal adenocarcinoma is a prominent example of cancer characterized by frequent amplifications in oncogenes. However, the mechanisms leading to amplicons that involve breakage-fusion-bridge cycles and extrachromosomal DNA are poorly understood. Here,
Alvin Wei Tian Ng, Dylan Peter McClurg, Ben Wesley, Shahriar A. Zamani, Emily Black, Ahmad Miremadi, Olivier Giger, Rogier ten Hoopen, Ginny Devonshire, Aisling M. Redmond, Nicola Grehan, Sriganesh Jammula, Adrienn Blasko, Xiaodun Li, Samuel Aparicio, Simon Tavaré, Oesophageal Cancer Clinical and Molecular Stratification (OCCAMS) Consortium, Karol Nowicki-Osuch, Rebecca C. Fitzgerald   +18 more
doaj   +1 more source

TRE5-A retrotransposition profiling reveals putative RNA polymerase III transcription complex binding sites on the Dictyostelium extrachromosomal rDNA element. [PDF]

PLoS ONE, 2017
The amoeba Dictyostelium discoideum has a haploid genome in which two thirds of the DNA encodes proteins. Consequently, the space available for selfish mobile elements to expand without excess damage to the host genome is limited.
Thomas Spaller, Marco Groth, Gernot Glöckner, Thomas Winckler   +3 more
doaj   +1 more source

Disparate Pathways for Extrachromosomal DNA Biogenesis and Genomic DNA Repair. [PDF]

Cancer Discov
Abstract Oncogene amplification on extrachromosomal DNA (ecDNA) is a pervasive driver event in cancer, yet our understanding of how ecDNA forms is limited. In this study, we couple a CRISPR-based method for ecDNA induction with extensive characterization of newly formed ecDNAs to examine their biogenesis.
Rose JC, Belk JA, Wong IT, Luebeck J, Horn HT, Daniel B, Jones MG, Yost KE, Hung KL, Kolahi KS, Curtis EJ, Kuo CJ, Bafna V, Mischel PS, Chang HY.   +14 more
europepmc   +5 more sources

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

Small extrachromosomal circular DNA (eccDNA): major functions in evolution and cancer

Molecular Cancer, 2021
Extrachromosomal circular DNA (eccDNA) refers to a type of circular DNA that originate from but are likely independent of chromosomes. Due to technological advancements, eccDNAs have recently emerged as multifunctional molecules with numerous ...
Xiaoxuan Ling, Yali Han, Jinxue Meng, Bohuan Zhong, Jialong Chen, He Zhang, Jiheng Qin, Jing Pang, Linhua Liu   +8 more
doaj   +1 more source

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source