Results 71 to 80 of about 2,048,993 (218)

Hungry for Knowledge: Octopamine Signaling Regulates Hunger‐Enhanced Olfactory Learning

Advanced Science, EarlyView.
Researchers demonstrate that hunger state facilitates both aversive and appetitive olfactory learning. Two distinct octopamine signaling pathways are involved in aversive or appetitive memory formation in the hunger state. And, hunger state also facilitates the formation of both types of memories via an evolutionarily conserved norepinephrine (the ...
Huijuan Zhao, Guiyuan Shi, Ruixue Qin, Yinghao Sun, Wenbo Guo, Ruixia Shi, Minxian Peng, Jingxuan Yang, Jianjian Zhao, Qiuhan Liu, Jun Xiao, Ke Zhang, Qiang Liu, Wenxing Yang, He Liu   +14 more
wiley   +1 more source

Engineered Stable, Antibiotic‐Free, High‐Level Protein Expression in the Probiotic Chassis Escherichia coli Nissle 1917

Biotechnology and Bioengineering, EarlyView.
The probiotic E. coli Nissle 1917 was engineered to enable stable and antibiotic free protein expression platforms using native plasmids. Further modification aids in the production of high‐level protein expression for antimicrobial secretion whose activity is comparable to industry standard E. coli BL21.
Halimatun Sakdiah Zainuddin, Sanjeeva Kumar Murali, Thomas J. Mansell   +2 more
wiley   +1 more source

Extrachromosomal circular DNA is common in yeast [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance We performed a screen for extrachromosomal circular DNAs containing segments of genomic yeast DNA. We found 1,756 such extrachromosomal circular DNAs containing about 23% of the total yeast genomic information. The abundance of these circular forms of genomic DNA suggests that eccDNA formation might be a common mutation that can ...
Møller, Henrik D., Parsons, Lance, Jørgensen, Tue Sparholt, Botstein, David, Regenberg, Birgitte   +4 more
openaire   +3 more sources

In Vivo Cytoskeletal AMPA Receptor Transport Imaging in C. elegans

Cytoskeleton, EarlyView.
ABSTRACT Long‐distance intracellular transport of ionotropic glutamate receptors (iGluRs) is essential for proper excitatory synaptic function underlying learning and memory. Many neuropsychiatric and neurodegenerative conditions have abnormal iGluR transport and trafficking, leading to an intense interest in the mechanisms and factors regulating these
Michaelis A. K., Hoerndli F. J.
wiley   +1 more source

Extrachromosomal DNA Transformation of Caenorhabditis elegans [PDF]

Molecular and Cellular Biology, 1985
DNA was introduced into the germ line of the nematode Caenorhabditis elegans by microinjection. Approximately 10% of the injected worms gave rise to transformed progeny. Upon injection, supercoiled molecules formed a high-molecular-weight array predominantly composed of tandem repeats of the injected sequence.
D T, Stinchcomb, J E, Shaw, S H, Carr, D, Hirsh   +3 more
openaire   +2 more sources

Extrachromosomal DNA : New Players in Oncology

Asian Journal of Medical Sciences
DNA that is found outside the main chromosomes in a cell's nucleus is known as extrachromosomal DNA. This type of DNA is not part of the standard 23 pairs of chromosomes in humans and instead exists as standalone circular or linear DNA structures. Unlike
Ruby Dhar , Arun Kumar , Subhradip Karmakar   +2 more
doaj   +1 more source

An Improved Transformation System for Molecular Biology Research in Peach Fruit

Future Postharvest and Food, EarlyView.
Schematic Diagram of Repeated Agrobacterium‐Mediated Transformation. A triple‐stage on‐tree Agrobacterium injection enables gene manipulation in peach with up to 32‐day expression, supporting efficient, nonheritable functional analysis without tissue culture.
Chunhong Li, Yijia Xia, Minghua Zhou, Fei Xiang, Wancheng Huang, Xuansheng Tan, Yanyu Zou, Kaituo Wang   +7 more
wiley   +1 more source

Human Smc5/6 recognises transcription-generated positive DNA supercoils

Nature Communications
Beyond its essential roles in ensuring faithful chromosome segregation and genomic stability, the human Smc5/6 complex acts as an antiviral factor. It binds to and impedes the transcription of extrachromosomal DNA templates; an ability which is lost upon
Aurélie Diman, Gaël Panis, Cédric Castrogiovanni, Julien Prados, Bastien Baechler, Michel Strubin   +5 more
doaj   +1 more source

Inactivation of p53 rescues the maintenance of high risk HPV DNA genomes deficient in expression of E6.

PLoS Pathogens, 2013
The human papillomavirus DNA genome undergoes three distinct stages of replication: establishment, maintenance and amplification. We show that the HPV16 E6 protein is required for the maintenance of the HPV16 DNA genome as an extrachromosomal, nuclear ...
Laurel D Lorenz, Jessenia Rivera Cardona, Paul F Lambert   +2 more
doaj   +1 more source

Rapid reconstruction of multidrug‐resistant bacterial genomes using CycloneSEQ nanopore sequencing

iMetaOmics, EarlyView.
Multidrug‐resistant (MDR) bacteria commonly harbor highly complex genomes enriched with mobile elements and resistance islands, which are challenging to resolve using short‐read sequencing. Here, we evaluated CycloneSEQ, a newly developed Chinese nanopore sequencing platform, for MDR genome reconstruction.
Kai Peng, Zeyu Tong, Changan Li, Zhichao Li, Xin Lu, Cemil Kurekci, Mashkoor Mohsin, Zhiqiang Wang, Yong‐Xin Liu, Ruichao Li   +9 more
wiley   +1 more source