Results 81 to 90 of about 2,048,993 (218)

Extrachromosomal DNA in Chloramphenicol Resistant Myxococcus Strains [PDF]

Journal of General Microbiology, 1976
The presence of extrachromosomal DNA in strains of Myxococcus xanthus and M fulvus was examined by rate-zonal centrifugation of radioactively-labelled DNA in 'cleared lysates'. All the strains examined contained extrachromosomal DNA, with the exception of M. xanthus FBt. Chloramphenicol resistance is inducible in M. xanthus FBt.
N L, Brown, J H, Parish
openaire   +2 more sources

Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum

Nature Communications, 2018
Genetic tools for research on red algae (rhodophytes) are lacking. Here, Li and Bock present an efficient genetic transformation system for a model rhodophyte, and show that the transgenic DNA can be maintained as an extrachromosomal multi-copy plasmid ...
Zhichao Li, Ralph Bock
doaj   +1 more source

Disparate pathways for extrachromosomal DNA biogenesis and genomic DNA repair

, 2023
ABSTRACTOncogene amplification on extrachromosomal DNA (ecDNA) is a pervasive driver event in cancer, yet our understanding of how ecDNA forms is limited. Here, we couple a CRISPR-based method for induction of ecDNA with extensive characterization of newly formed ecDNA to examine ecDNA biogenesis.
Rose JC, Wong IT, Daniel B, Jones MG, Yost KE, Hung KL, Curtis EJ, Mischel PS, Chang HY.   +8 more
europepmc   +3 more sources

Synthetic Biology and Metabolic Engineering of Microalgae for Sustainable Lipid and Terpenoid Production: An Updated Perspective

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microalgae are increasingly recognised as powerful platforms for the sustainable production of lipids and terpenoids, with expanding applications in the food, fuel and biomanufacturing industries. In this updated review, we consolidate and critically assess the most recent advances in synthetic biology and metabolic engineering of key ...
Ty Shitanaka, Yu Wang, Sally Do, Julia Yuson, Samir Kumar Khanal, Krzysztof Zienkiewicz, Zhi‐Yan Du   +6 more
wiley   +1 more source

A mechanism of gene amplification driven by small DNA fragments.

PLoS Genetics, 2012
DNA amplification is a molecular process that increases the copy number of a chromosomal tract and often causes elevated expression of the amplified gene(s).
Kuntal Mukherjee, Francesca Storici
doaj   +1 more source

HAM-ART: An optimised culture-free Hi-C metagenomics pipeline for tracking antimicrobial resistance genes in complex microbial communities.

PLoS Genetics, 2022
Shotgun metagenomics is a powerful tool to identify antimicrobial resistance (AMR) genes in microbiomes but has the limitation that extrachromosomal DNA, such as plasmids, cannot be linked with the host bacterial chromosome.
Lajos Kalmar, Srishti Gupta, Iain R L Kean, Xiaoliang Ba, Nazreen Hadjirin, Elizabeth M Lay, Stefan P W de Vries, Michael Bateman, Harriet Bartlet, Juan Hernandez-Garcia, Alexander W Tucker, Olivier Restif, Mark P Stevens, James L N Wood, Duncan J Maskell, Andrew J Grant, Mark A Holmes   +16 more
doaj   +1 more source

Transgene‐Free, Gene‐Edited Cavendish Bananas (Musa acuminata, AAA)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Global consumer acceptance of gene‐edited food crops is increasing with new breeding technologies that can modify the genome without foreign DNA integration. Here, we report an Agrobacterium‐based system for transgene‐free, gene editing of the banana cultivar, Cavendish.
Maiko Kato, Jen Kleidon, Amba Phillips, Alexandra Tabrett, Robert Harding, James Dale, Jean‐Yves Paul   +6 more
wiley   +1 more source

How a retrotransposon exploits the plant's heat stress response for its activation.

PLoS Genetics, 2014
Retrotransposons are major components of plant and animal genomes. They amplify by reverse transcription and reintegration into the host genome but their activity is usually epigenetically silenced.
Vladimir V Cavrak, Nicole Lettner, Suraj Jamge, Agata Kosarewicz, Laura Maria Bayer, Ortrun Mittelsten Scheid   +5 more
doaj   +1 more source

Deregulated enhancer‐promoter communication in cancer through altered nuclear architecture

International Journal of Cancer, Volume 158, Issue 2, Page 409-422, 15 January 2026.
Abstract Enhancers are critical regulators of gene expression. Structural variations in cancer genomes can lead to enhancer hijacking, where oncogenes are activated by mistargeted enhancer activity. Novel enhancer‐promoter interactions may also arise through chromosomal rearrangements that create extrachromosomal DNA elements.
Isabelle Seufert, Claire Vargas, Sina Jasmin Wille, Karsten Rippe   +3 more
wiley   +1 more source

Circular DNA elements of chromosomal origin are common in healthy human somatic tissue

Nature Communications, 2018
Somatic cells can accumulate structural variations such as deletions. Here, Møller et al. show that normal human cells generate large extrachromosomal circular DNAs (eccDNAs), most likely the products of excised DNA, that can be transcriptionally active ...
Henrik Devitt Møller, Marghoob Mohiyuddin, Iñigo Prada-Luengo, M. Reza Sailani, Jens Frey Halling, Peter Plomgaard, Lasse Maretty, Anders Johannes Hansen, Michael P. Snyder, Henriette Pilegaard, Hugo Y. K. Lam, Birgitte Regenberg   +11 more
doaj   +1 more source