Results 51 to 60 of about 504 (141)

Characterisation of a cold‐adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS‐131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation

open access: yesThe FEBS Journal, EarlyView.
We investigated glucokinase from the psychrophilic Pseudoalteromonas sp. AS‐131 (PsGK), which was isolated from the Antarctic Ocean. Biochemical studies revealed that PsGK is a cold‐adapted enzyme with high thermal stability, in contrast to general cold‐adapted enzymes, which have low thermal stability.
Akane Yato   +10 more
wiley   +1 more source

Pulsed Electric Fields for Emerging Single‐Cell Bioprocessing in Food Applications: Electropermeabilization Mechanisms and Design Principles

open access: yesComprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 2, March 2026.
ABSTRACT This review evaluates pulsed electric fields (PEF) as an emerging platform for single‐cell bioprocessing in food applications. Connections are drawn between key mechanisms in electropermeabilization and applications, and a practical PEF process design framework is provided.
Byron Perez   +4 more
wiley   +1 more source

Airborne Halophilic and Non‐Halophilic Microbial Communities in an Underground Salt Mine Affected by Seasonal Environmental Fluctuations

open access: yesEnvironmental Microbiology Reports, Volume 18, Issue 1, February 2026.
This study tests seasonal controls on airborne microbes in the UNESCO‐listed Bochnia Salt Mine. In summer, relative humidity exceeded the halite deliquescence threshold, coinciding with higher NaCl aerosol and greater densities of halophilic microbes; in winter, humidity stayed below deliquescence and halophilic microbes declined at least 13‐fold while
Aleksandra Puławska   +9 more
wiley   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

open access: yesFEBS Letters, Volume 599, Issue 23, Page 3417-3443, December 2025.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

A polyextremophilic alcohol dehydrogenase from the Atlantis II Deep Red Sea brine pool

open access: yesFEBS Open Bio, Volume 9, Issue 2, Page 194-205, February 2019., 2019
A polyextremophilic alcohol dehydrogenase (ADH) was identified from single amplified genomes of the Atlantis II brine pool and was characterized in terms of activity and stability. The ADH was stable under high temperatures and hypersaline conditions, tolerated organic solvents, and preferably converted cinnamyl alcohol derivatives.
Anastassja L. Akal   +7 more
wiley   +1 more source

Mining Thermophile Genomes for New PETases with Exceptional Thermostabilities Using Sequence Similarity Networks

open access: yesChemBioChem, Volume 26, Issue 12, June 16, 2025.
Targeted genome mining identified thermostable enzymes (PETases) that hydrolyze the commonly used plastic polymer polyethylene terephthalate (PET). Thermostable enzymes are identified from thermophilic microbial sources. Experimental results reveal enzymatic activity on authentic PET films, highlighting potential for industrial application.
Zhenyu Hu   +4 more
wiley   +1 more source

ProteoSeeker: A Feature‐Rich Metagenomic Analysis Tool for Accessible and Comprehensive Metagenomic Exploration

open access: yesAdvanced Science, Volume 12, Issue 19, May 22, 2025.
ProteoSeeker is a new metagenomic analysis tool that accelerates protein discovery. By automating key steps, it reduces computational complexity and enables comprehensive dataset analysis, performing protein identification, screening based on user‐defined protein families, and taxonomic analysis.
Georgios Filis   +6 more
wiley   +1 more source

Utilization of formic acid by extremely thermoacidophilic archaea species

open access: yesMicrobial Biotechnology, Volume 17, Issue 9, September 2024.
Our work explores formic acid metabolism in extreme microorganisms, novel hosts that hold promise for biomanufacturing distinct products including thermostable enzymes and lipids. We investigated formic acid tolerance and utilization in archaea species within the Sulfolobus genus, and novel bioreactor culturing methods to overcome formic acid toxicity.
Sara Tejedor‐Sanz   +2 more
wiley   +1 more source

Advances in Aureobasidium research: Paving the path to industrial utilization

open access: yesMicrobial Biotechnology, Volume 17, Issue 8, August 2024.
The extremotolerant fungus Aureobasidium chassis can utilize a broad spectrum of substrates to synthesize value‐added products including pullulan, polyol lipids, polymalate, and melanin. We furthermore describe available genetic engineering tools and up‐scaling approaches.
Difan Xiao   +7 more
wiley   +1 more source

Characterization of an extremophile bacterial acid phosphatase derived from metagenomics analysis

open access: yesMicrobial Biotechnology, Volume 17, Issue 4, April 2024.
Acid phosphatases are enzymes that play a crucial role in the hydrolysis of various organophosphorous molecules. A putative acid phosphatase called FS6 showed high sequence similarity to type C acid phosphatases and retained more than 30% of consensus residues in its protein sequence. A histidine‐tagged recombinant FS6 exhibited extremophile properties,
Maria‐Isabel Recio   +7 more
wiley   +1 more source

Home - About - Disclaimer - Privacy