Aggregate, Volume 7, Issue 2, February 2026.This study presents a dynamic‐responsive sensing system composed of RNA aptamers and polymers for adaptive histamine sensing. Programmable RNA self‐assembly via PEG8K enables phase‐separated condensates with enhanced stability and affinity. A label‐free FRET system (ThT→HBC dyes) achieves high‐sensitive detection (15.36 nM) in complex matrices ...
Yifan Wu +7 more
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New biosensors and transgenic mice for multiplex cGMP imaging
British Journal of Pharmacology, EarlyView.Background and Purpose Cyclic guanosine monophosphate (cGMP) is a versatile second messenger that is important for human (patho‐)physiology and pharmacotherapy. Live‐cell imaging of cGMP with biosensors allows to elucidate its spatiotemporal dynamics in real time under close‐to‐native conditions. However, to monitor two separate cGMP pools or cGMP/cAMP
Markus Wolters +6 more
wiley +1 more source
Monitoring lipid-protein interactions in planta using Förster resonance energy transfer. [PDF]
Methods Enzymol, 2023 Yao S, Wang X.
europepmc +1 more source
A quad‐cistronic fluorescent biosensor system for real‐time detection of subcellular Ca2+ signals
British Journal of Pharmacology, EarlyView.Background and Purpose The calcium ion (Ca2+) is a versatile cellular messenger regulating a variety of biological processes. Compounds modulating subcellular Ca2+ signals hold substantial pharmacological potential. Advances in fluorescent biosensors have revolutionised Ca2+ imaging.
Anna Lischnig +7 more
wiley +1 more source
Internal Calibration Förster Resonance Energy Transfer Assay: A Real-Time Approach for Determining Protease Kinetics [PDF]
, 2013 Ling Jiang +6 more
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Quantum dots for Förster Resonance Energy Transfer (FRET)
, 2020 Marcelina Cardoso Dos Santos +3 more
semanticscholar +1 more source
PDE4D and PDE3B orchestrate distinct cAMP microdomains in 3T3‐L1 adipocytes
British Journal of Pharmacology, EarlyView.Basal conditions: •Ins/PDE3B lowers cytoplasmic cAMP (cyt‐cAMP) without affecting plasma membrane cAMP (pm‐cAMP). •Insulin decreases lipid droplet cAMP (LD‐cAMP) independent of PDE3B. •FGF1/PDE4D modestly reduces both cyt‐ and pm‐cAMP, while PDE4D alone can modulate LD‐cAMP. ISO stimulation: •Ins/PDE3B has minimal impact on cyt‐cAMP.
Johannes Krier +9 more
wiley +1 more source
Fluorescent Probes with Förster Resonance Energy Transfer Function for Monitoring the Gelation and Formation of Nanoparticles Based on Chitosan Copolymers. [PDF]
J Funct Biomater, 2023 Zlotnikov ID +2 more
europepmc +1 more source
Simple Estimation of Förster Resonance Energy Transfer (FRET) Orientation Factor Distribution in Membranes [PDF]
, 2012 Luís M. S. Loura
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