Protocol for live-cell Förster resonance energy transfer imaging to reveal the bistable insulin response of single C2C12-derived myotubes. [PDF]
Akhtar J, Imran M, Wang G.
europepmc +1 more source
cGMP Signaling in Arterioles Revealed by FRET‐Based Real‐Time Measurement in Mice In Vivo
ABSTRACT cGMP evokes arteriolar vasorelaxation and is generated in smooth muscle by the soluble guanylyl cyclase (sGC) stimulated by endothelial NO. Phosphodiesterases (PDEs) degrade cGMP and may contribute in diameter regulation. We examined arteriolar cGMP levels in real‐time in vivo upon NO and acetylcholine (ACh) and effects of PDE inhibition ...
Lukas Thiele +6 more
wiley +1 more source
Förster Resonance Energy Transfer and Enhanced Emission in Cs4PbBr6 Nanocrystals Encapsulated in Silicon Nano-Sheets for Perovskite Light Emitting Diode Applications. [PDF]
Herrera Mondragon A +13 more
europepmc +1 more source
Bacteriophage P22 Virus‐Like Particles as Nanoscale Protein Scaffolds for Plant Synthetic Biology
ABSTRACT Advancing the utility of plant synthetic biology requires the continued development of protein engineering tools. Self‐assembling protein compartments, such as virus‐like particles (VLPs), provide versatile scaffolds for synthetic biology. However, few plant‐expressed VLPs have demonstrated broad amenability to protein engineering, restricting
Maxim D. Harding +5 more
wiley +1 more source
Modifying the conformational ensemble of “molten‐globule” protein by the site‐specific incorporation of stereoisomeric 4‐fluoroproline residues modulated its binding affinity and aggregation behavior. 19F NMR spectroscopy enabled the detection of key interactions responsible for structural and dynamic changes. ABSTRACT Recently, the application of deep
Abir Ben Bouzayene +5 more
wiley +2 more sources
CdSe/ZnS Quantum Rods (QRs) and Phenyl Boronic Acid BODIPY as Efficient Förster Resonance Energy Transfer (FRET) Donor-Acceptor Pair. [PDF]
Salerno G +8 more
europepmc +1 more source
Monitoring Hierarchical Assembly of Ring-in-Ring and Russian Doll Complexes Based on Carbon Nanoring by Förster Resonance Energy Transfer. [PDF]
Guo S +11 more
europepmc +1 more source
Click'n ROPISA: One‐Pot Synthesis of Ultrabright Fluorescent Nanoparticles Made of Polypeptides
Self‐assembly and fluorophore conjugation proceed rapidly in a one‐pot “Click'n ROPISA” process, yielding intensely fluorescent polypeptide nanoparticles with tunable visible emission. High dye loading enables efficient energy transfer and single‐particle visualization, while the straightforward synthesis provides versatile access to bright ...
Amin El Jarroudi Hammou +7 more
wiley +2 more sources
Shewanella oneidensis and Bacillus subtilis, both harboring prophages, reveal a novel biosynthetic route to scalable and programmable DNA superstructures in flower‐ and wire‐like shapes, respectively. Activation of prophages is achieved via 3‐hour starvation and monitored using reporter strains.
Gabriel Antonio S. Minero +7 more
wiley +2 more sources
Improvement of the Green-Red Förster Resonance Energy Transfer-Based Ca2+ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor. [PDF]
Matsuda T, Sakai S, Okazaki KI, Nagai T.
europepmc +1 more source

