Results 21 to 30 of about 8,470,173 (241)

A STING-based biosensor affords broad cyclic dinucleotide detection within single living eukaryotic cells

open access: yesNature Communications, 2020
Cyclic dinucleotides are conserved second messengers but current detection methods are limited. Here the authors engineer a Förster resonance energy transfer (FRET) based biosensor, BioSTING, which gives real-time in vitro detection of these nucleotides.
Alex J. Pollock   +2 more
doaj   +1 more source

Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats

open access: yesNature Communications, 2021
DNA three-way junctions are branched structures formed during replication, repair, and recombination, and are involved in models of repeat expansion. Here the authors use single-molecule Förster resonance energy transfer to reveal the dynamics of DNA ...
Tianyu Hu   +2 more
doaj   +1 more source

Investigation of FRET from organic dyes to silver nanoparticles and structural properties using the DFT/TD-DFT approach

open access: yesChemical Physics Impact, 2022
The Forster resonance energy transfer involving a fluorescence molecule and organic quencher is constrained to a distance of ∼10 nm. However, with the coupling plasmonic materials, a significant amount of energy transfer can take place over substantially
HR Deepa   +2 more
doaj   +1 more source

Conformational changes in ezrin analyzed by Förster resonance energy transfer [PDF]

open access: yes, 2015
Ezrin gehört zu der ERM Proteingruppe und seine Hauptfunktion besteht in der Verbindung zwischen dem Zytoskelett und der Zellmembran. Diese Arbeit demonstriert Veränderungen der Ezrin-Konformation während seiner Aktivierung und erklärt Synergismus der ...
Shabardina, V. (Victoria)
core   +3 more sources

Analysis of Enzyme Conformation Dynamics Using Single-Molecule Förster Resonance Energy Transfer (smFRET)

open access: yesBiophysica, 2022
Single-molecule Förster resonance energy transfer (smFRET) enables the deconvolution of various conformational substates of biomolecules. Over the past two decades, it has been widely used to understand the conformational dynamics of enzymes.
Mai Huynh, Bhaswati Sengupta
doaj   +1 more source

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

open access: yesMicromachines, 2021
Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale.
Yi Qiao   +4 more
doaj   +1 more source

Förster-type resonance energy transfer (FRET): Applications [PDF]

open access: yes, 2016
In this chapter, we present several applications of Förster-type nonradiative energy transfer (FRET) related phenomena. In particular, we review light generation and light harvesting applications as well as bio-applications. © 2017, The Author(s)
Hilmi Volkan Demir   +5 more
core   +1 more source

T-cell Receptor Is a Threshold Detector: Sub- and Supra-Threshold Stochastic Resonance in TCR-MHC Clusters on the Cell Surface

open access: yesEntropy, 2022
Stochastic resonance in clusters of major histocompatibility molecules is extended by a more detailed description of adaptive thresholding and by applying the notion of suprathreshold stochastic resonance as a stochastically quantizing encoder of ...
László Bene   +2 more
doaj   +1 more source

Live-cell Förster resonance energy transfer imaging to reveal the bistable insulin response of C2C12-derived myotubes

open access: yes, 2023
To ensure safe and rapid glycemic control, muscle insulin response must exhibit bistability. We utilized single-cell Förster resonance energy transfer (FRET) imaging to quantify the bistable insulin response of C2C12-derived myotubeTHIS DATASET IS ...
AKHTAR, J (via Mendeley Data)   +1 more
core   +1 more source

Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances

open access: yesNature Communications, 2018
Current Förster resonance energy transfer (FRET)-based fluorescence spectroscopy methods are suffering from some limitations in the field of structural biology.
Antonios Pantazis   +4 more
doaj   +1 more source

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