Results 21 to 30 of about 6,735,254 (244)

A STING-based biosensor affords broad cyclic dinucleotide detection within single living eukaryotic cells

Nature Communications, 2020
Cyclic dinucleotides are conserved second messengers but current detection methods are limited. Here the authors engineer a Förster resonance energy transfer (FRET) based biosensor, BioSTING, which gives real-time in vitro detection of these nucleotides.
Alex J. Pollock, Shivam A. Zaver, Joshua J. Woodward   +2 more
doaj   +1 more source

No better time to FRET: shedding light on host pathogen interactions [PDF]

, 2010
Understanding the spatio-temporal subversion of host cell signaling by bacterial virulence factors is key to combating infectious diseases. Following a recent study by Buntru and co-workers published in BMC Biology, we review how fluorescence (Forster ...
Leong, J.M., Goguen, J.D., Goguen, JD, Leong, JM, Hayward, RD, Hayward, Richard D.   +5 more
core   +1 more source

Resonance Energy Transfer [PDF]

, 2015
Resonance energy transfer, also known as Förster- or fluorescence- resonance energy transfer, or electronic energy transfer, is a photonic process whose relevance in many major areas of science is reflected both by a wide prevalence of the effect and ...
Bradshaw, David, Scholes, Gregory, Andrews, David, Dinshaw, Rayomond   +3 more
core   +2 more sources

Analysis of Enzyme Conformation Dynamics Using Single-Molecule Förster Resonance Energy Transfer (smFRET)

Biophysica, 2022
Single-molecule Förster resonance energy transfer (smFRET) enables the deconvolution of various conformational substates of biomolecules. Over the past two decades, it has been widely used to understand the conformational dynamics of enzymes.
Mai Huynh, Bhaswati Sengupta
doaj   +1 more source

T-cell Receptor Is a Threshold Detector: Sub- and Supra-Threshold Stochastic Resonance in TCR-MHC Clusters on the Cell Surface

Entropy, 2022
Stochastic resonance in clusters of major histocompatibility molecules is extended by a more detailed description of adaptive thresholding and by applying the notion of suprathreshold stochastic resonance as a stochastically quantizing encoder of ...
László Bene, Miklós Bagdány, László Damjanovich   +2 more
doaj   +1 more source

Förster resonance energy transfer within single chain nanoparticles. [PDF]

Chem Sci
Single chain nanoparticles (SCNPs) are a highly versatile polymer architecture consisting of single polymer chains that are intramolecularly crosslinked.
Maag PH, Feist F, Frisch H, Roesky PW, Barner-Kowollik C.   +4 more
europepmc   +2 more sources

Fluorescence resonance energy transfer studies of protein interactions [PDF]

, 2008
This thesis presents an investigation of fluorescence resonance energy transfer (FRET) as a reporting signal for protein-protein interactions. Quantitative optical assays to measure protein binding, conjugation and deconjugation are developed and results
Martin, Sarah Friede
core   +1 more source

Förster resonance energy transfer photoacoustic microscopy [PDF]

, 2012
Förster, or fluorescence, resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1 to 10 nm range.
Lihong V. Wang, Wang, Yu, Yu Wang, Wang, Lihong V.   +3 more
core   +1 more source

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Micromachines, 2021
Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale.
Yi Qiao, Yuhan Luo, Naiyun Long, Yi Xing, Jing Tu   +4 more
doaj   +1 more source

Förster Resonance Energy Transfer (FRET) Demonstrates In Vitro Chitosan-Coated Nanocapsules Suitability for Intranasal Brain Delivery. [PDF]

ACS Appl Mater Interfaces
Intranasal drug delivery to the brain offers a promising strategy to overcome biological barriers. Chitosan-coated nanoemulsion-based nanocapsules demonstrate significant potential due to their mucoadhesive properties, ability to permeate epithelial ...
Alleva M, Baranyai Z, Esteban-Pérez N, Martínez-Vicente P, Martín-Rapún R, Moros M, Martínez de la Fuente J.   +6 more
europepmc   +2 more sources