Results 41 to 50 of about 8,470,173 (241)
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Dual Förster resonance energy transfer was developed in a PTB7-Th (donor)/P(NDI2OD-T2) (accepter)/PF12TBT (the third component) ternary blend system.
Zhiyuan Xie +5 more
core +1 more source
We review the use of self-assembled, vertically oriented one-dimensional (1D) titania nanowire and nanotube geometries in several third-generation excitonic solar cell designs including those based upon bulk heterojunction, ordered heterojunction ...
Ningzhong Bao +2 more
doaj +1 more source
Revealing Nucleic Acid Mutations Using Förster Resonance Energy Transfer-Based Probes
Nucleic acid mutations are of tremendous importance in modern clinical work, biotechnology and in fundamental studies of nucleic acids. Therefore, rapid, cost-effective and reliable detection of mutations is an object of extensive research.
Nina P. L. Junager +2 more
doaj +1 more source
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley +1 more source
Förster Resonance Energy Transfer Measurements in Living Plant Cells
Schmidtpott S, Seidel T. Förster Resonance Energy Transfer Measurements in Living Plant Cells. Journal of visualized experiments : JoVE. 2021;(172).Sensitized emission-based Forster resonance energy transfer (FRET) experiments are easily done but depend ...
Schmidtpott, Sonja +3 more
core +1 more source
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
The photoexcitation energy transfer is found and investigated in complexes of CdSe/ZnS cationic quantum dots and chlorin e6 molecules formed by covalent bonding and electrostatic interaction in aqueous solution and in porous track membranes.
Irina V. Martynenko +5 more
doaj +1 more source
The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap ...
Kaleeckal Harikumar +5 more
doaj +1 more source
Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source

