Results 51 to 60 of about 8,470,173 (241)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Enhancing the organic solar cell efficiency by combining plasmonic and FÖrster Resonance Energy Transfer (FRET) effects

, 2021
Here, we combine two strategies i.e., Förster resonance energy transfer and plasmonic effect, to enhance the photovoltaic performance in organic solar cells by introducing gold nanoparticles and squaraine in a binary mixture of poly(3-hexylthiophene ...
김동하
core   +1 more source

Electroporation and microinjection successfully deliver single-stranded and duplex DNA into live cells as detected by FRET measurements. [PDF]

PLoS ONE, 2014
Förster resonance energy transfer (FRET) technology relies on the close proximity of two compatible fluorophores for energy transfer. Tagged (Cy3 and Cy5) complementary DNA strands forming a stable duplex and a doubly-tagged single strand were shown to ...
Rosemary A Bamford, Zheng-yun Zhao, Neil A Hotchin, Iain B Styles, Gerard B Nash, James H R Tucker, Roy Bicknell   +6 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

No better time to FRET: shedding light on host pathogen interactions [PDF]

, 2010
Understanding the spatio-temporal subversion of host cell signaling by bacterial virulence factors is key to combating infectious diseases. Following a recent study by Buntru and co-workers published in BMC Biology, we review how fluorescence (Forster ...
Leong, J.M., Goguen, J.D., Goguen, JD, Leong, JM, Hayward, RD, Hayward, Richard D.   +5 more
core   +1 more source

Förster resonance energy transfer within single chain nanoparticles. [PDF]

Chem Sci
Single chain nanoparticles (SCNPs) are a highly versatile polymer architecture consisting of single polymer chains that are intramolecularly crosslinked.
Maag PH, Feist F, Frisch H, Roesky PW, Barner-Kowollik C.   +4 more
europepmc   +2 more sources

Circularly Polarized Electroluminescence and Spin‐Polarized Carrier Transport in Chiral‐Doped Polymer Films

Advanced Functional Materials, EarlyView.
Chiral dopants induce supramolecular chirality in an achiral polymer (PFO), generating spin‐polarized carriers via the chiral‐induced spin selectivity (CISS) and suggesting a possible contribution of Spin‐polarized carrier transport to circularly polarized electroluminescence (CPEL).
Kazuyuki Asahi, Masashi Hasegawa, Yuuya Nagata, Tamaki Nakano, Hiroyuki Nishikawa   +4 more
wiley   +1 more source

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1.

, 2019
Bulk methods measure the ensemble behavior of molecules, in which individual reaction rates of the underlying steps are averaged throughout the population.
Bralic, Amer, Takahashi, Masateru, Hamdan, Samir, Tehseen, Muhammad, Ouyang, Yujing, Raducanu, Vlad-Stefan, Rashid, Fahad, Zaher, Manal, Sobhy, Mohamed Abdelmaboud   +8 more
core   +1 more source

Managing Charge Transport and Exciton Generation for Highly Efficient, Bright, and Stable Near‐Infrared Organic Radical Light‐Emitting Diodes

Advanced Functional Materials, EarlyView.
High‐performing near‐infrared (NIR) organic light‐emitting diodes are demonstrated using an efficient and stable 800 nm‐emissive organic radical and a thermally activated delayed fluorescence (TADF) host. TADF‐assisted exciton generation combined with improved charge transport enables high external quantum efficiency (>8%), high radiance (>70,000 mW sr−
Hwan‐Hee Cho, Min‐Hsuan Chang, Yi‐Syun Chen, Tetsuro Kusamoto, Emrys W. Evans, Yuh‐Renn Wu, Richard H. Friend   +6 more
wiley   +1 more source

Fluorescence resonance energy transfer (FRET) in chemistry and biology: non-Förster distance dependence of the FRET rate [PDF]

, 2006
Fluorescence resonance energy transfer (FRET) is a popular tool to study equilibrium and dynamical properties of polymers and biopolymers in condensed phases and is now widely used in conjunction with single molecule spectroscopy.
Singh, Harjinder, Saini, Sangeeta, Bagchi, Biman   +2 more
core   +1 more source