Results 51 to 60 of about 6,735,254 (244)

Tryptophan-Tryptophan energy migration as a tool to follow apoflavodoxin folding [PDF]

, 2008
Submolecular details of Azotobacter vinelandii apoflavodoxin (apoFD) (un)folding are revealed by time-resolved fluorescence anisotropy using wild-type protein and variants lacking one or two of apoFD's three tryptophans.
Mierlo, C.P.M., van, Westphal, Adrie H., van Amerongen, H., Visser, N.V., Amerongen, H., van, van Hoek, Arie, Hoek, A., van, Visser, Antonie J.W.G., van Amerongen, Herbert, van Hoek, A., Visser, Nina V., Westphal, A.H., van Mierlo, Carlo P.M., Visser, A.J.W.G., van Mierlo, C.P.M.   +14 more
core   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Self-Organized One-Dimensional TiO2 Nanotube/Nanowire Array Films for Use in Excitonic Solar Cells: A Review

Journal of Nanotechnology, 2012
We review the use of self-assembled, vertically oriented one-dimensional (1D) titania nanowire and nanotube geometries in several third-generation excitonic solar cell designs including those based upon bulk heterojunction, ordered heterojunction ...
Ningzhong Bao, Xinjian Feng, Craig A. Grimes   +2 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Energy transfer in complexes of water-soluble quantum dots and chlorin e6 molecules in different environments

Beilstein Journal of Nanotechnology, 2013
The photoexcitation energy transfer is found and investigated in complexes of CdSe/ZnS cationic quantum dots and chlorin e6 molecules formed by covalent bonding and electrostatic interaction in aqueous solution and in porous track membranes.
Irina V. Martynenko, Anna O. Orlova, Vladimir G. Maslov, Alexander V. Baranov, Anatoly V. Fedorov, Mikhail Artemyev   +5 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells

Bio-Protocol, 2017
The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap ...
Kaleeckal Harikumar, Yan Yan, Ting-Hai Xu, Karsten Melcher, H. Xu, Laurence Miller   +5 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Electroporation and microinjection successfully deliver single-stranded and duplex DNA into live cells as detected by FRET measurements. [PDF]

PLoS ONE, 2014
Förster resonance energy transfer (FRET) technology relies on the close proximity of two compatible fluorophores for energy transfer. Tagged (Cy3 and Cy5) complementary DNA strands forming a stable duplex and a doubly-tagged single strand were shown to ...
Rosemary A Bamford, Zheng-yun Zhao, Neil A Hotchin, Iain B Styles, Gerard B Nash, James H R Tucker, Roy Bicknell   +6 more
doaj   +1 more source

Förster-type resonance energy transfer (FRET): Applications

, 2016
In this chapter, we present several applications of Förster-type nonradiative energy transfer (FRET) related phenomena. In particular, we review light generation and light harvesting applications as well as bio-applications. © 2017, The Author(s)
Hilmi Volkan Demir, Demir, Hilmi Volkan, Govorov, Alexander, Hernández Martínez, Pedro Ludwig, Alexander Govorov, Pedro Ludwig Hernández Martínez   +5 more
core   +1 more source